	   SnapGene      acgttatcgactgcacggtgcaccaatgcttctggcgtcaggcagccatcggaagctgtggtatggctgtgcaggtcgtaaatcactgcataattcgtgtcgctcaaggcgcactcccgttctggataatgttttttgcgccgacatcataacggttctggcaaatattctgaaatgagctgttgacaattaatcatcggctcgtataatgtgtggaattgtgagcggataacaatttcacacaggaaacagtattcatgtcccctatactaggttattggaaaattaagggccttgtgcaacccactcgacttcttttggaatatcttgaagaaaaatatgaagagcatttgtatgagcgcgatgaaggtgataaatggcgaaacaaaaagtttgaattgggtttggagtttcccaatcttccttattatattgatggtgatgttaaattaacacagtctatggccatcatacgttatatagctgacaagcacaacatgttgggtggttgtccaaaagagcgtgcagagatttcaatgcttgaaggagcggttttggatattagatacggtgtttcgagaattgcatatagtaaagactttgaaactctcaaagttgattttcttagcaagctacctgaaatgctgaaaatgttcgaagatcgtttatgtcataaaacatatttaaatggtgatcatgtaacccatcctgacttcatgttgtatgacgctcttgatgttgttttatacatggacccaatgtgcctggatgcgttcccaaaattagtttgttttaaaaaacgtattgaagctatcccacaaattgataagtacttgaaatccagcaagtatatagcatggcctttgcagggctggcaagccacgtttggtggtggcgaccatcctccaaaatcggatctggttccgcgtggatccatgagtggaggatctcaagtccacattttttggggtgctccaattgctccactgaaaatcacagtatcagaagacacagcttctttaatgtctgttgctgacccctggaaaaaaattcagcttttatacagtcaacattctttatatctgaaggatgaaaaacagcacaaaaatcttgaaaactataaagtcccagaatctattggttctccagatcttagtggtcatttcttagcaaactgtatgaatagacatgttcatgtgaaagatgactttgtacgttctgtttctgaaacacagaatatagaatcccagaagattcactcctctagactgagtgatataactagctctaatatgcaaatatgtggatttaaaagcacagttccgcatttcaccgaagaagaaaagtatcaaaagcttctcagtgaaaataaaattagagatgaacagcctaaacatcagccagatatatgtggtaagaactttaacacaaatttgtttcagttgggccataaatgtgcagctgtgttggatttggtttgtagtactgaaaaaattaatatagggcctgaagtggtacaaagagagtgtgtgccaacagaatatcatgaaatacaaaaccagtgtttgggattattttcctcgaacgcagtagataagtcaaggtctgaagcagcagttaggaaggtctcagaccttaaaatatcaactgatacagaatttctcagtataattacctccagccaggttgcttttttagctcaaaagaaagataaaaggcggagtcctgtaaataaagggaatgtaaacatggagactgaaccaaaggcaagttacggggagataagaatacctgaagagaattcgattcagcttgatggttttacagaagcatatgaaagtggacaaaaccaagcatattcccttgaactttttagtcctgtttgtcctaaaacagaaaatagccgcattcacataaactctgataaaggtcttgaagaacatacaggatctcaagaacttttcagttctgaagatgaactgccaccaaatgagatacgtattgagttgtgtagctcaggaatactgtgttcccaactaaataccttccacaaaagtgctattaaaagaagctgtacctctgaagataaagtgggccagtctgaagctctatctagagtccttcaagtagctaagaaaatgaagttgatttctaatggaggagattctgctgtagaaatggatcggagaaatgtgtctgaatttaagagtattaaaaaaacatcattaataaaaaactgtgattctaaaagccagaagtataattgtttagtcatggtgctatctccatgccatgtgaaggaaataaacataaaattcggaccaaattctggctctaaagtgcctttagcaacagttacagtaattgatcaatcagaaactaagaagaaggtttttctgtggaggactgcagcattttgggcatttacagtgtttcttggagatataattttactcacagatgttgttattcatgaggaccaatggattggcgagacagtactacaatcaacatttagcagtcagttattaaatcttgggagttattcatctattcagcctgaagaatattccagtgtagttagtgaagttgtacttcaagacttactggcatatgtgtcctcaaaacattcctacctcagagatcttcctccgaggcagcctcagagggtgaacagtatagactttgtagaattggagcaccttcaacctgatgtattagtccacgcagtactaagagttgttgatttcactatactgacagaggcagtatacagttatagaggacagaagcagaaaaaagttatgttaacagtggaacaggcccaagatcaacattatgcgcttgtattatggggtcctggagcagcctggtaccctcaacttcaaaggaaaaaaggttatatttgggaatttaaatatctttttgttcagtgcaattacacactagaaaacctagaattgcatacaacgccttggtcatcctgtgagtgcttgtttgatgatgatataagggcaattacatttaaagcaaaatttcaaaaaagtgcaccctcctttgtgaagatatcagacttagcaacccacctagaggataagtgttcaggagtggttctgattaaagcccagatttcagagctggcatttcctattacagcatctcagaagatagcgctaaatgctcacagttctctgaagagtattttttcttctcttcccaacatcgtatatactggttgtgcaaaatgtggattggaactagaaacagatgagaacaggatctacaaacaatgttttagctgcttgccatttactatgaagaaaatatattataggccagcgttaatgactgccattgatggaagacatgatgtttgtatccgtgtagaatcaaagctgatagagaagattcttctcaacatttctgcagactgcctcaacagagtgatagttccttcctcagagatcacctatgggatggtcgtggcagacctgttccactccttgttggcagtcagcgcagaaccttgtgtattaaagattcagagcctttttgtgttagatgaaaacagctatccattacaacaagatttctccctcctggatttttatcctgacattgtaaagcatggagccaatgcccgtctcGAGCAAAAGCTCATTTCTGAAGAGGACTTGtaagcggccgcatcgtgactgactgacgatctgcctcgcgcgtttcggtgatgacggtgaaaacctctgacacatgcagctcccggagacggtcacagcttgtctgtaagcggatgccgggagcagacaagcccgtcagggcgcgtcagcgggtgttggcgggtgtcggggcgcagccatgacccagtcacgtagcgatagcggagtgtataattcttgaagacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtgttgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgcagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcataaattccgacaccatcgaatggtgcaaaacctttcgcggtatggcatgatagcgcccggaagagagtcaattcagggtggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtgcgatcttcctgaggccgatactgtcgtcgtcccctcaaactggcagatgcacggttacgatgcgcccatctacaccaacgtaacctatcccattacggtcaatccgccgtttgttcccacggagaatccgacgggttgttactcgctcacatttaatgttgatgaaagctggctacaggaaggccagacgcgaattatttttgatggcgttggaatt  B               #   %   &      #            $                      %   <   8   !      *   -                      &   %               &      %   !   *                  (         %   &   &         0               $   "                  "   5   *      $      !      +   $   )         9      &   2   Y   8   -   +   "   ,         1   #      "   ,   *         ,   &         *   1   !          #                  2            )               #            /   &   0   !   @   .      &      /         "   !      	               0            $   +                  "   #   %         '   $       (   &   K   0      &   0   (               *   '         +                                                                            0   +   /      /      +   !      $                  4   5                           #   !      &                     %            #      "                        Æï%ŽÌÍæJKLðñABC Èðé	[‘Ó�Îé«åòþÃ} �öÇk
&óýsÌMÍŒ¤©¶ !d¤Þ,r	ÄçAþçADAÿ‘ÄW‡£5DÉ# –!­·Öñloˆ`	†	ê	‡
- -±Ô'¦±ðt½e¬ØæH—),ª% 2 Ö ô ?TÉâûÿ
I\µ�œúêÒóQ {ÿ.Â$B~ ã& { â ,þ‘¡°?¼ šƒ´	°Ì’ÌŽQÏpz%ŸW/¯´Äÿ0Z ï1	c	¬	ß
V©‡¶#  pµ÷9±wzÈãl
‹”ôþ�UtÃD¦HV��¾Û¥°³à¯>_ ; „é7›èÕðZu»Âý1g¼†½ ™ ‘ú&—sÂ”U	Q%¡\q€-©KNo7o¶f· I …BXˆ' –·Í=Î ª’$ ! k z}´¹	èúãEw 5³E>W‰ÉÝF’Ù"�ˆ;tˆ¹ôûÿ  ( Ÿ iås	0
_
Ì
ÅCœ´„.ÿU)^Él2BðXE­Êö[ @ Æ e	¡¥|óB èì¤ÿ(Ü "ÏZ¥ß-bB�˜è²dv�äkÅ6˜cÉ>ç ®ma	ª	]
i
Ê
Ý
T§uãþUÇ< Aañ×òÇp‰‡	¼	P
ÅI…²Ñ§b>}”­Ù« —©öó¸ÓCžë	°
«!|q(þ{©1~ÔÅò�µ*�ä-% ° Þ Xd"ˆ
.>Ø.?ÕÊ²ñ¾Æä^†f�Ûç&IÉéú
˜Ð,< 3 IUm�&ì<á69äGU"Ž²!6\ÎøO	R	

ä
õ[yô KQ7ûh¶®ú©r¨|	ÄÏ/€ÓAÄä8 « õ L}Z~†CÜCX®Ê<TÏ©+gV~‹üG	ê
J„¼÷ë—=‘ôMXC-õ–¯ôR™ö bgÃC!Õ! × @‹CjœÒÙwÊùã–
‚
]�:$döR“Q%ð÷­ñ f‰.ÝI‘£¸o
 ¶ÇÁ�‰¹ëH)ÀFjxÓáÞÀ* j²¸Þÿ@²cµ›	—
·ºÂ(“èŽÍbÇRqí&6¬¹ Si~_lÒü- | ã s-48ú{8q­ô‚É$‰	½ø^Eu!Jhß‚{´@¶�%0°ÎqµÅÍ1B iä½!brÀµ	¸
+7±NÒOƒ-§SŒ œT[  “´’¢±|›„>	R
Í‡xk� ¢Ðâ7 ¬úXœyÓ3 Õu‘´:zø	"ƒÊ³ì Ž ¸ åï¡2¾	Æ
à
#¡ÚÓZœ3·IaÖ²  5TÆµòÙd	(
y
ÿdWmq(l!�%¾y ð€
_	­	 ªˆeÁìnåÌ\N& 9 Ô øx{Ÿ«ƒ‚ç/*^	ý
#®š‚}áÊIñþ­ŽÙ0®±ý,•Á"% Ü q¶²¬øR¾ö
m
©óskŒ•HV€Ä§Ò‹4Çø‘—Ü?`Îâ*w ` Ò :·õ÷Øê�}	
7
5Èâõÿ*ó£¿´   D (
øÉ¯ùä#	t­å¥–unES-W¦á,°/ Ç5ÚN{

7ò¥«Ç½¾ M Oº…¿Î8	$\Æ\œ^Öñ[vÃ Ö¼�& < Ë û `Ñ!Oæ˜A�Î Ñt
š
Ð
~˜/Ê,Å éÖý¼þ·2h·o =»ÊêÂ	
©
83‡% µ óûfÎá¡¹•Õ_ž	
²
µ
¢±÷põ.æ2�MZp¬P•ä¸Ï!  »ÚPê2	¡	Ü	í	
×
ñ
ö
Rä]�®ê)�¥I¾àíæ‹g»* ‚ ‡’™'<—äsÄ›ÃXÙV		³Û+†r©>«½ªkÖLPÐî8·jž v š Œ˜t¦¿'­ìo&ry¥×ÜOp‘AÏ^¢ J b —>Ï*¯Jz„¶Ì¡gtHK $ �)†=ÞØ	¡¸¸ãíc}=aè=ôƒù«“ É CY‰2’:ÂÎÁgVÔÞäW þ¹dºS\E9
 
(š	3ŸN( à $µ`Êñû%š,™æ‹xÓF;´ÇFb/eü?‡ÇÛÞÂ²<Ç. h{~º	FhðüôŠ‹#gÂõ1ï " �
„ëÈæ‰(›×.X¬Ê÷G“Üâª“‰º % l ‰ fb¼‚é˜ä5’ˆ6šÝëuu—ð¹Ú‚‰�’¨u‘Òçüo& ) F a±2JMh	Õ	`
¦
Í
ç
0a�µ¾¨×	k!„ÞƒŠ8ÊÛí²§Ë é\&   *sKj‘æ,	vÆÕ§A $*7_-]YC}ò²H‹)F€ÔÙ W ZHV,%=qk	t	1
’DS)f/HVU'>mš¤ñY   n êK"Š
ç…wÌÃáÊUß3qã9®÷£Îb0 6 A ² âƒf´x@áBRepó¢¦±;&ô÷C~ËP)2»éíNl„¥&Mª¶%)Ù w)	QædòÜ´?`á�IÝ  õJ˜¯ä¾æ»W)}a¡é" þ e Ç ØËÔ¡Ý9	kG«ËD”Ü9‰ÔÚêÂ ‹ ncÐûÀT¨ØÞ»w™ê™d?›$ , Ðnw¦û�½.Êñè0sŽýé³úœYåé]¨#.s”7²è" #ú[kà%	ý	c
3›hÚ}CO6ÝGÏùKew‚¿Ò5‡›ì 26ß±™õ\wlÆSwÊ×q  cÝÇj
Ãæ@ªÌäï@‹V4C ó  ¯>S™ánúb	«	Þ
HËUù¨°†ž ×ÿþAâ H oAW“‡T	P[€C¥tºµ ' j ³ä^
Ë
v±4²ã!:õþÈ=æ" W`ðòB�O
h
¯
É
Ü
={Hp„×Sý¨a]…Ú	~€´Æ5 KBëiÑØà8;ˆv¨ø!f�¢U}ŠÍF	N	Q	
�
ã
é
ƒŒZ»Æö9JÀL¸#Bcô¿•®¿f¬* 3’{±ãbz7¬ó´È#qˆ	š	Q
÷6]ÁM†ÑçajŸì6?~«ùŒ~}^ Sî ñ^Ø½	'
x
þ"cŸ³Ù9yÒk2dë!Ë`‚Õë$ ª‚÷Qæô.)"	|	

ü
s¬ä"r)|m¢ðýÆ÷ü+ÀÍ. ú N<Nå@É4€MŸÐ7	Á	s
¨
Ï
}ÿ[2]Ÿ¶n! †˜e& Oé¬Ô^1	Û	ì	

±
Ö
ð
qxoªÖ€jÕ­(Îåã È¸(c<¹R1DÝ×	Ÿ
·<ó‚Ifž+ ß e#a	»_Éð$˜ÇåE‡:gˆ™ÆÜ'êïût«ÆÚÁÆÑ-Á$ YérJUJ�!IL$<+	j	Ô	‰
æ
/ÔR(@ÖËG#àT‚É\™±¢) 5 ± áv(ôI³ Ü8P?àAñFª½³òV_`ŽèÊ1ÙL©éHµ$Ø + mjv�mÉü	b
21Ù/Þü¿ô§[JÝèÑë i n=@V²Æã†ùS	GOÂ?ø¯�ÿs3©± Bå9 JVR‘í°â7:‡ï§«÷�¡³"TœM	P	
&
®

ö\zõ!LÐR8ü`iž·`ªø\¾ }�ÔeÅ …d;M·b?å;è-(L]Ï!	
ï
ãZÕiÍ& 4 èu'l HH#;7Èå
Ó0@'E^ÈÝFûóS˜Å
ÅÐÙ0Å#×2 „Qca¯>Gî¦ª±Åâ,…'Ç 	î
FNÒâ/?Ï&D>÷Y_ÔE~œ÷þ—°ØdÄ"ÖY MN‚ƒ‡¢
‹ÝDEOPY=FUZÐÑÒe¥°5ahW¥¦§¨¶·¸Æ	?
ë
ì
í
KLM…×ßàá©øìC˜õö^Dˆ›Åö3456ST[÷chi€8 O ¢ ¬ Ov¾ˆ­3DŒÞQV[•«ÓfÁiÃöXŒý		â	cØgªù’µ¿â·.Æ“B�´õJ- ö ~[£FZ“ËoÐ6b{©¹	H	@
†½Ôí™N9‰/7õU\šøEAúj‚D�V+   ­ Ï w€‰®õý¬ËÄYi€�±	h«Ø3`n“ãÄ%÷&S”>KR�¤ßñøÌ" £ AP�Ó	WØgÂújä÷³þ
dúk¶ÀÖëîh/”ö{¾, [ » Ø é Œ¿D4Ð\–Ôx…—Õ			:	Ž	ƒ
^xŽÈÙ0ed–C†€	Û&7ò P ¿  E�ßRkÚB	É	ã	;¸¹Ç!ä‘çµH—–® – /˜OºŸ/*ÏGköV_â¢ùÁôvÖq~1 æ ÷ Ãî¤,GÌgp�þÑc’ª	.	Í	A
p
ù
’¾ÈÕXmîþ•…Ç,a0Ô7Fk>û4Eà# \[”ÿŠmJ|¤¹òºI	@·Š‘Ø:AI]ŠfxÁy: +’W ,¼Þ7’
‡ÂšžÙx·ì8³]›ß*Bðkƒ‚ƒ" > x³Í¯íXÔj�á²	#�”+oSÊ'rî#OTjºòùý, ® SbkÓ§Aš³d	)¶ÅZ¨	¸»Ãò&é�fÏÅÈ&X|ø'¿’o~SLRÓ* € Ð ¹ñŠ›¸Ìßöþ¤ª€­ÌŽôœ	5
˜
ô
¬Ù)a”ÃßÔ�•­?T1ÞàÍ  � ïi4¦Îäci7¹!FLjŽ¥`m ßLSžÖÛkv
Ú
_Fv"Yi0A&r¿, Y } ” Í ä t 9_ýX©9ƒ®Ãõyƒ%´Óÿ	Œ	À.il×àìvƒ|µ·1±@ó2& ¤ BQ­Ý.J§Q6Ôû
|~Ùh'?Š	
3
Ue¾ùšûýÁïsu÷¶ã 
Ö5é$rû•ÊåøÁ
Ò
”œK· wŽ•Õ€Ï¨ÆÎ|C Ù �¯E#5ÑpÐÖ;	¶	„
�²!ñ®ÛW€T�&¦'* ¼ ªÀ0]Õ"†¢°ß	œ
 yÉ,MÿÌùëf>eP¹�qÊ—¡ÜòU�ó1 ê \j©¿‰.—¾%yc˜m	v	�	Ð	¹
W_ÎÚ8F€ë[KS¥­1„�—.4Lt8Èö! \ ¦ ý /åAssÁ
		¤	ð	ø	
D
OÓ1» ¨D‡$
�\  / Ã ”Ž…•h†£ãÿ8O¶[˜Ô  Q ‘ À ÓµàèCsj­?	C	Ê	û<ˆlÑØûYÈ�hz÷I# D¡“²8Sl}œ>éä	ô	S
gUÎy�J¹¡º" )’èœù¸4u Á,F„£Û¤1õÇ¡î­Èå¾ å§ˆ—¯  — 7í’µOHm„Xd�¨´ v;y 
+#láãªÂwÈË°j   Øq½Ì{»0Ð£ìr Ö0¤
™MPùŸ÷W`%úsõ‰~×í2 ñ ø Æãò&HÏ$hÿtd—/	5	z	˜	¿	q
Ç
Ô
á
ú
Po¿Énï»–@[4Y†¸ÕGl×ü³?Fáà ¹ ç €æì1M“ì3Ç	–ÒÖüÿÛÔ�Å-b158å?5o � ï¥ë½-¢qÒw“«	Î	B
Ì“$Y¤¹„ÈÓ�"¥Æ ï ÄðÍ‘|~¢†Ws9Jb=y) 	 T Ù•óÌnÚºç»e	-
Ã
ženr‹ˆBmãL^áy—z;¡Ž&d\¿`zEX \ óJ	Ä	«
¾
€¸ï;J ; 6UÇ?¶‹K¥!
)
z
A X‡Î)’–MŒy‹¹"“ p 1]é}”	ª	5ÙQogÂþ,î¾ 3«¸í<Ì,u´¼Ñæ;Í+šÐ„Ð#  �ß8À	®	9y‰fÂ9Bc�¶íLœà]®CñôlO{ z`‰ýúJ
Œ
“
ˆ‹¡Ã›×o‚^`„ -½ñ:éŸÚHídoùƒŽ �õÅ£+q	è	$Z�r~ËèËå£þ�¨†Â|/ z Õ >•+îý ¬»Õ0k‚_	…	³	†
þ
,¯´,�os¼$dP®-–(Á#+& : ´ù°vy–r„ƒÁèâ6Ÿ$ •pT,¨ïYJüU�Ú¯²»Ÿn®^0   ? ž yÎYh|	¸	/
B›³ùƒâT(D¤{òÿu€k1=Vjçëï€s—¬b‡¸óú'ZÛþ! ¯ Ý c¨õÆÒ©	ªÄÐ¦É0}Å=Óã
|Œ“%ÄÈùTÏã+;@ ª HTlŠY}…%BÛ;BW›­ÉSeÎã
FÜ *H�±· 5[•÷ 

n
ôxóêP–6óWgõù(¨E’˜ÝP@aïÔ. r±·h³Ý7ùp�a¿=	–
·
¶¹¼*'Dt�IgÞÆ�á³N‚µ5¦ÍpRRSÑû · äÅÔ	´Å
lpt� ÷'Y›À [Mx& 8 C Ñ Ó Û '÷«�®¶öø×é�½õ]	6
¨ò¤­Çá•à3¬,–+á!) _¹Ð—ºÆ¾ÍÙz

~
™
.6É#[Å+ÄÕ¤ÆÕ/ � ´ ºò;‹Íàã¥Ãš¸WëØ~	�	 	´
õ
n²Ú*¤°ãö¨=ôOé�@½ßO¡ºÎ a ýœÿ[‘8
Á b`™®ÀƒµUÓ2G ˆ —+Š4‘š.óáf�§±æ0n"   E V )G`°g	u‘æ¦`§vjÂ ƒÝ,TXpâ8G(¦Ÿa!  ‚ÊÓ—wå®jãåCdÛo°%(àö8}ˆO(Mk
% ùú¼ð$	çg5|Ï5rFSùº\§"4-rR	 ÜRZB¤°ô 2Ÿ×ð_6A|w
€
Û
‚‹º8Gæxj1}ó¾ M�ÈQÜ

ž
w¡¬'¿ àqT�ŸlÛO*	Ó	`ß#U¦ÈsÀèÀ :†:öS›L	­
Á]Ç�_òR2ÄÌ0 N u½P¡¬`Ynªd¯ÀÄÏ`z„õ&¤µÅ 			á	bÑ.fÎ%´á]Ó}¶‡šA�×ô Z ~ ¾ Î ü×„h²Ô9	�	/_jmíg•¸ ã=£Þ–½Ë • å Â»þ�ø
Øw„¶×@\Îw²¡
ï3‚ = RaÌšÒ™(4
ó
"ñŽÂÞh"‘Ni¸K$ Ì 	¬ÜÞKèPR#5^Õü}Ú‚Ò‹	u
À
Ñ
Ù
T¿™›ü-Xtv§â+ ¥ ü ©®/¨"o$@Ï¡£	Þ	ï	÷	
›
VLþËR¤¬ðê=­¸3Kpsõ  C+ç7Bi=èfú0ôÆí×þ½ÿŸÇø·ä‡ ×6¼ìËLNl$©ˆÇ}Wi� ê%ës–4	y	Æ	Ó
{•ËOÑû¸ƒÒr…!nxß >Ëæ“	Ã	 
,
ª
Â
�î4•LPnx–D½ yˆüùI
9Ab�Öì+�»Ð™Ï�" =Ž”ô*uÄq¢ºÀp	„	·	ç	…
Yž³²øq+çXcU§­–# ¶ G‰°XgÜ$6AVGŒÑ`<	¶
µ5ò¥ö/à�´	$ÃQÐú% Ú &^üÏâ¹™ÅZÖ×Ÿ	
}
³
m±ñ£-¯Æà"ZÀ§N2‚( ¹ ‡ F_Ò–�æ*4BÁ3‚¯Ü+R|‡N['q$Qžå 1p€Ž^QžPÛ)	Ò	
�
Þåw|¯¿çó' ½ ¼Á«ûŽÖcš£±à	÷
aÐ-Í^Ò?fQ†”™ÍŽ	Ëî¢Ý•Ê$  ¨«Û™ç"4#¢	Ý	î	Ø
ò
!S W«ìg¬ž¦ÆJrá
ô  ‡ë3	x	’	+
zÊNíú‚M”Omë*kº# ˜VmŒhŽ¼( † <]ˆ“)W^tÃ¸˜¿ÄYo	|
Xl´ð,ß¦)3AÀ³MQ1{†Â Ÿù& ˜ªæú!3��O™Ú(	w	‘	Ñ	`ÏÝìVªÑ«l{…“˜j	lÉK ƒ „ … ;(kåÀÁÂ &š›œMN§W	X	n	�	jk�‚ƒÜÝÞTU‰®¯&'(2?@¬¾Ð½¾¿‘’±²×kl/0hiz…u9k÷ø0 ë š=lŠÅ/œ'z¨dY	€	º
Û9G„\L‡sŠ°­¿Øm•5aÑïMv:¸9®	 ] >�		ˆ‹®ÙPb¯& ì þ É§æ0�t©I¥	ñ	ù	#
�
»
CpÜ:Ô¼è	�»6ˆ;ž:]0  w › § 8m÷0u‹ÀÆL{®etÂ	Z	�	's…ˆPS]M2t±Ø˜Žž’©ðëNwÐ( ›¤™((Bíý	
E
V
H�z~¦!9ÀY�Ýn£ëq–EÒ%B_¹Û
7£ 0 Ä �†	‰Œ¤–?Ð×»  3?Ž,
=‡+P·Í K ^ É •–˜iFä Ú	Q;K\huIL c €¯ÛÑ9ÚÖc°{…•î¢· ™°ÕÀ* % éOtž\~ªßD	Ù	ú	M
f
qü;X‚¹Õäî~m!bé€>õ‰Éú{2J' g aµ*D>k£ŸJ@	Ë	$
=
�
>DÝ=Vk½é
“üZ¼„ži<øT” ’ Á ÿ Ôž¶Ê‡¨®ß¦	ò	¢IdÍÒÙqŽ½ÜŸð R í áç1u–	¼
m‰¹F7>J‹7¬üZì;^Ä+  x œ ¨ ”9ÁÇD¯3u“	[	(v^NzžÃÙ™�º#ÄÞª*ñhY�Ñßå Êmî;?fÃ	õ	Ï²S	¢Ÿv 9E¢nÚø1T~ŒŠ‚	å	T
h†‹Q3uKi“ÄéO5 _³vMZ|�êVt‰T;‘Ï»Â“ìWxÕú¹.0XŠ š)Z
2Èžo¿FC4 ˜° ÿGˆ¤ºÍÖxŒþì

¡
§öÁÞìc  ¥Â…ë)»T]F:
F
W
)¥íI{¬:¢®›æ
r— 8 r œ-eÜCîŽ	­"ïZùÉ¤¡Ó&z`æŒº¨ÜO‰¤ îÒòü�ý@Iý@Ã¢Èµ 1 á �¶‡	°Ë&›P¥ïyV.µÑGz³/Y©  ˜ %8Š“¶šçÂÔG<Án0f½ß…6¼=e‘ Å a-Œ]— ÈcˆÈÜEØ @»	\
>zâèñýœ |�5-<ˆµ­G,qiw§àQõÃÎÒÃöð iw ¡$,«‹›¸hxÌ›Ò2Ak  � 4mäHŒ$É±½² _ 	ž4™jGòÉÑ
RŠØ/­”v L Ê  Í—üñª¶»€  u Ù–r¾…¼å1Ûœ¤ÏíLYH]«ì”iŠ� # 9âì|ç)<yËøÝãVfsJM gœ�ƒê™åÒadÖû-t†–ïöŠ¸Û‘©�’î m +1p¥
½e6‰7ÞÚì&=±|ñ£ŠÓØÍè d ×cÊQº“:>Û Óºƒ!ýÁ Š šN°úÜÎ›øX¾×vv˜“év†š±Öpš & G ò b’˜àÊå…¹ËÖî?Šâý39´ý@á0 êhzÐßy ]u eÌE	™	N
g
È
â
è
ý5<YÀboƒž¿ÖØK"Aë5cê„‹³Ê_|]ê+ ù Çä'%PŸ3N«00	6	{	À	Ö	Ú	
r
§
Î
Õ
û
rp¶n;\lïüöžHeûmÌâ/ * IóIiu²KŽi	û	a
1Qºð©¼ï
—"ßD�9Z_‡[Üîçª°ØK¨G¡´ê h çtLk¶?ç: K%
	 Üh[½/ ¡ �b	2ŠN’E”í¤á4Â	>
EÖÞ¨Ó>B¾8Äý.2’ZD~³@Iù�U  º è ŸôÏA	°	È	wÇ×2ÕêÃ%c…æŒGzÚ6•+ +í+ýlñ-	Ì	‘
?—‘Wlý”�žÆ+.`e^)69Ÿój=u*Õp‘ß}!  ð·ËìW¦£©Óàîó§	%*¥eÎW{ÉÓ¾Žn}0EÝQ X “ ÕIŸ¦¨&>x”	2
“uÿrÔ?ò$ [¾.ˆ-r¯l	u	Ï	C
ÍEêZø Jº0ÚV�#%¥É– ñŒÇ . � Â ÒÀƒr¬ó	£T”g…I(Ng›Zö¦t  p£
xžàër¯c§ î ð Åë0¼âñLÎ#’v —	}n£º?ÄVXŒ84ä<>¤Å S o 0Øè½
©†Í‡‹âKà¸:ýc[í_  2¿‹
èŠG8;µËt:K_c­ónøÏz4  y � üg•	.
+5AŸSŒCn£§»äzût<i¢�žæêî'm…]r«Àa{†·&*YÚ5 © ã|„ÄÚ:ÈôÍâEoÛÿ€°4”ÿ	•
Ä
)wCOosŸÝfšô²<MâD{¥ÌQZOî: 7 B ³ –Âµ½Vèy¼\	§£Ä<_˜Åè«+Ô*¼ÒNF 
 U »vf	â_f{Úq‰�$'ßõø™òo37àe¦	' ï@�Š¹T£  m_ôRgÄ	K	¬
e³µâ< Ë*]n
<çö	¿
Kð�óQ£Ý<!�MJ† xÅ	H
ÐK
@aÑq„wÞ %FÛ@UF‹ƒ	æ	�°å®ÅY¿4¤*ß*b#P#� §o�b*
vL“NÌ"Å”# :V]ù2·Œ™L¦'	{
iïÜˆ%Ï¼z°jgy„”Á`­ÿ!  7£ÈöKüH"
U
Ž
B‡ŒR}ç8—X�œŒ~�º¢êêÚ6 È 2+<E
Ú•ÐÙ:Õ”íÖÿº¿& f `´N[}¢žÞ•	<
L
e
=WlUj�HÌE’ 6Ip¼ŠûÛ1YëïSÃ ^Ùí~:	uŠ�RhÃÝÃX-/‰ q ‡ÌÕwê‹ëY
ì«¬øby‹ ¯Ñüî\ŸÁU-Ày¼D5¡ Œ 4[
´¬pv¦ßçGš›Ñš¼Ñ@± t 38áñûð©µÎxëUeœ ›*odÉ¹=Í½ÒÙ<Õ,è …�Ì™% HgyÞx�‘/Ë
4J:êCîû^íZ�áæd©^¯8ü³é  ž ó‰Îüà9Áð	¯	�1Â�gÃd‘(ty�Þ| - Ñ¿‚¥§íòé÷tzþM$f|È/D•õPs‹ //o»×¾ÿ¢

¨Šß:´·�ê^bòm¦# {Ãûá¼þUþ	”
*4¦¢Ü;çús˜¤nÓ6q…í9 l^G
‰JŒP¢ÐpƒL…" $¦\aEûG&	�
œî|­ÄÛ$7£Þ›¯ËœfxƒÀ!_œ¬Ù †ì*Š;
K
X
d
<ëi€~œD÷HØaˆR s 7®à³ð[úï¥xÔçTŽ»Ë .�¾ò.fÝï	3�I]'e{Çaxû�ÝrŠ¥ D;ê
¡Û#šöÊ£mp„«ØQ  ²î¢�ú©ªP  -®  qGACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,GCACCCANNNGTT,AACNNNTGGGTGC,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,CACCCA,TCGGTC,TGGGTG,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        	                                                                                                               "                   	                                                                                                                 %         
      	   	      
             "         	                  
   )                                                      (      )   %                !   n   w      "   ,         '   ^                                                                                                                                                                                                                                          
                                                                                                                                                                                                                                                                                                                    	                                         "       	                                         %   
  	 	  
     "   	      
 )                  (  ) %      ! n w  " ,   ' ^   }×  ~Ñ  ~á    ‚±  ‡§  �õÚ!	M\ò	
®©ÀfxM›�R ï†¹4B~$*5ã™ìu]
„²e aË˜k	’bµ!™YË²lÒ)²¹‚‰‹wË÷Îìõ¯	(©$»&
¸ ±qŒ¸ ±qÆ¸ ±q±qq±[£¨ u%Ecq4q&o‰qÔ&Ç:§ï^bt'`'5Xx©_9ÝUª Ý&@*±‡„¢y��h©R
3)­)&zÐ
4æF 7<ÉÁ	¨
27D¥töÉÒÞ ",º/¢ÎaŒ—b•w–		]ØB:âEÿ°4ÿ	wOf~! zøÉtaï¢�õ Éˆ
åuÊSá åuSá<Ä¼ Õ°e¡Ü� åuSáÄÎ¡ÄxàEtì 7€Ó/Å¼Ä³=	ã¡Žzyf	_Å„Þa™t�tû{Q
ï—¡&7-œê½æDHo¥Ätö+»'*w‹b¤#f ´ž¡ÒXÍË[
œípû„¦qí,¿4$‹Ú%À»�mêa]„D 
¯
 o7a¡<äÒ9V² Éˆ
åuÊSáÄ¦	dÍ½ï¢�Ð
œŠUyQû|Äsfj@ä¢)Y~Ôå?q¨5Âéí�"ªÇ²¶Ê%Ù¦ö
íï¢¤�Ë‘¡°{šQ
ÌŽŸŸ«hgkËS ‡cª`ÏÑ…˜´w³E˜øµ:âEÿ°4ÿ	+wOf»'*w‹~bK�v$õ	&2
µ )ö
õíH µ ¹)Î/m
 
Ð
ö
åuõkSÜáíx E?ÂÚTxîŠŸß?¦	$
;i3FdÍº½Z¼¤Oºu8ÏõF°¹Ï²ï—D9K¨´•Ö<f	_²K¨´f ´ž<¤#£ 
îªåGcõr£<ÉÁ	
¨
2wïÛî?‘¡°šƒÌŽÏŸW¢ ¬û‘*4íúsá8qü…Y:ÎÁVÔW×¼	²Ñ) ±Jh	`
0á¨	p!Þ&ô÷8Ûí2¥&§ é!ï¤Þ,	¬	'A©‡˜çkÄÅ5É®Ñü[x¡pµ±l
‹”UÃ¦�Û>_1‰F7¬ï	¬	'©‡) ±Jh	`
0¨	!Þ8Ûí§ éáp&ô÷2¥&- Ñ¿‚Mfõs²“Rqí¹~ü¨wêd›) ±Jnh	`
0á`c¨	,p‹!Þ&ô÷8ÇbØÛí‡ÇÛ2¥&§ éû‘*4®Ñü[íúsxá¡8qü…SfH'šYÿÍb6 i_l-
ž‹Bmãy;¡Ž&\¿`zX/
:îû�dþ?’ÌQp%Àƒ£¦N[}ÞL
e
W� 1ô<â{:dN — ø Æ7ã&•$ç»/	5	z	¿	q
Ô
ú
o’µ[^—m´ÍGl„áEd�o�ôN[}ÞRK	L
e
¬
W�v <â{1:P{	
û
rlïüöûÌS Ø‡âKàc[_Õøì" ëæ(Ê÷ÜâÔçŽËñûü?ÂÇjÙä¸CrÀ:NÒ§ò��œ[­)yËÉfsJ²0 ¨ �é9ÇD\¯3†“	D	M
Z
f
ü(;Xv‚ÕNž!™¤béoÐFÉY{°ûG�
|7›œÙm<UøR	®r¨Aäºê2	k¸�=Ö[vê) «w‹g�»IU�ìá69Ž²!O	
õ[yô KQ7ûh¶©|€ÓÄ¢T
†‹Qé5¸Cò��k¸=«wjÙärÀ:NÒ§œ[­n`c,‹ÇbØ‡ÇÛS ØP{	
û
rl‡âKàïüöûc[Ì_SkAšd	)¶Zòéø'~Óþ É§¥	ñ	�žOX¨2gœ�ƒê™åÒadÖû-t†–ïöŠ¸Û‘©�’î›*odÉ¹=Í½ÒÙ<Õ,è …�Ì™à $µ`Êñû%š,™æ‹xÓF;´ÇFb/eü?‡ÇÛÞÂ²<Ç.=»ÊêÂ	
©
83‡5 ± áv(ôI³ Ü8P?àAñFª½³òV_`ŽèÊ1ÙL©éHµ$Øl ‰ fb¼‚é˜ä5’ˆ6šÝëuu—ð¹Ú‚‰�’¨u‘Òçüoï@�Š¹T£ “´’¢±|›„>	R
Í‡xk� ¢Ðâ7 ¬úXœyÓ3"ÏZ¥ß-bB�˜è²dv�äkÅ6˜cÉ>ç¯Ñüî\ŸÁU-Ày¼D5¡ ð·ËìW¦£©Óàîó§	%*¥eÎW{ÉÓ¾Žn}0EÝQ+ 5 @ ± Æ "Ïáv(ZemôIjv¥³ß� mÜ-8PÉ	ü	b
21?àAÙñ/Fbª½ÞB�³òüV˜¡¥¿èô_|§²óB[`ŽèJdv�ÊÝäèkÑ 1Åèì¤Ù6L˜©éÿHcµÉë$(>ØÜç= X “ Ì  	Ra¬ÌÕÜÞðIKšŸ¦·ËÒèìPRW¦#5^™£©Õü}¨Ú‚Ó&(>x”Òàîó	‹	§	2
4
u
À
Ñ
Ù
ó
"T“¿™›ñü%Ž*¥-XeÂÎÞWhu{ÉÓÿ"rtv¾Ô‘NŽ?in§¸}ò0EÝâKQ  Øq½Ì{»0Ð£ìr  E V )G`°g	u‘æ¦`§vjÂ ƒÝ,TXpâ8G(¦Ÿa[ » Ø é Œ¿D4Ð\–Ôx…—Õ			:	Ž	ƒ
^xŽÈÙ0ed–C†€	Û&7ò†ì*Š;
K
X
d
<ëi€~œD÷HØaˆR®ma	ª	]
i
Ê
Ý
T§uãþUÇ<½ ¼Á«ûŽÖcš£±à	÷
aÐ-Í^Ò?fQ†”™ÍŽ	Ëî¢Ý•Ê  5 H ± oØávz(qô½I³AW`‰“ ý‡ÌÜú8PT	J
Œ
“
Pˆ?àA‹¡ÃñF›ª½[{€»³ò0VC¥Ð_t×`o‚ŽºèÊ^£ì`1ÙL©µéHrµ$„Ø 3?Ž,
=‡+P·Í  .©     ¤          r›7ßXëƒ-=°Sˆ&#BÉ[xÙk"ÎÔ3{ò¸bªŒñÛeÂ¸5f¸VŠim°ì™b Z!0eÖ­ÝtšØ¿æy
hi@ú#_ÞÇ®¨n…Y…j&1…e`ÅuT¨Z J¿[¯¦™¡‚¥iwK˜=¶r™“‡SuØ  ¢õQ24F­c¡A0ªiÂ*ÄZ6d•¡^íR†«¤ÔçáXáV+l©‹e]š•‚b_Ûáëb‰‡;¡Š*™ØÖ#àc+õZJŠ¥UÉ¡NoÝZIèVôÔ™“`ú�ƒSÜ¨ ƒo½VÁ¸+Ì�½„�íKÝª ïZ;ÞmÒmwNº–.(áÿ�UZÓªgK]®¦nUtÝUºy•/Ñnh+½¤‚ÚºM^&Gb¶!Q‡¨œ®*á]ÑfE¿EÞû‘á!™y;–cVb¥St¯6­ñEIµS®ÒeZDatù]´ôfb	Eê]Õ¨«JÙX�8Š ¨’xUZY�>‚uDHˆõÑfØVu_H¨š¯±sIKP¸SM)´~•f×†tZ»I–Ÿ½=
:ªNÕEFUø••bW`G…õCRœEŸ…kÓ¡‚©ÑM˜T�Õ}Mšþ…ê¤¾*/•tU“Í­Ù^á•¡EÙÐnÔ^«JáF�}XFrhJ"NÐYâ+õågëÝTŽiTS‰û…RÒáNæäKëRNQV¡«–®8‘XV"ájQ&•UÛieUâåSÑI–Š’Ø#›½�b•ÙV°}ZàîT�©5Ò�%¡µ´W”QBªâ4Ú 6§H« Feª{tbŠkaÖƒÒyÖ×jM-)¥n€JknkO…kÒ$¤…(žµž˜‹íWkçíºûÄ4ûD]&G¨‘h~µøbi/sIåŠ·™áÑ&t¦DÑUJb—HCõ]¦3¢i€/�4ø'û^µAUB™¨©Yº¨­#Zwy~E£g\úoˆHè¨a†eZv¥MV­UHßï¨…´zM~DH­‚¸iSôjÑ86¯štÛ´Q“9XíÒ»…j«®ëõÛ&g‚ˆÕb�yÑ„]çWXªN:=©æU™¦CÓ)aãÚv¨›È‘mS†DQ®»]«�>×D†JúûDmù€`²Š÷¾Š-3Eú"iQ­ê¢)UÓ›ÚuÔjïï�ª›áÚ%M�=còîgŸFœéL<Û!ááÆãìÌ«aÂ~áÝ�;ðGtè¸”Á�4u?-2Ó(0"À3Oaý-É1“‰–ºqP>Èg>j¦B–-†Y`«¤rÐƒz¬XŒÁæ¢¦ªågkVbo;aeþXëYf…TI„š×jò,þšþªŒ6¨úøªŽßÑ\à…ITc…J7�±xµÓ	Fú*³üQr«ÖMê•+ŽbÂi¿"‡<D×°³Í�æ»E‡ ¡'·ÒÝMºp`Øt”E¦4Ž=—ØHeÞ0õéë‡\l|PN_:ª7]€Ø—³èl Á8XSÙõqÄÈwÜcãM`×\£5y¥àÅç§¹:ü5Ö–G��R�Þ¸ÎÃúðàà©£†VàOL€»0›hÓxÑ‚Sûòl’›�Ç KC^`aqY8FB>ÞSh%âÑõ*{fg©•zÚ©jUä!Fú¨e»aõ[ú\„ªÊ÷„ËGü‘TmPnº¾ª®3%¸ã£WUWß9ôÂ
Š�ÔN}{ª¼P—ƒ­4LÑŸVx¾¹"O$¤=÷­Eî$ß>v~Îã–ø§Ò8ôƒK"éð(Ôq’ŸPÍ0°8\ +#wOÓ L\~wÅáùÓ!9„TÏs¯Å	¼%0Ð,A7¯@sà›©’ø°*Ï~áèL±ªˆc²Ð Á>`UsÓ\Ãê§
ø>¨àú£·bº¾2›þâ_&Ÿ>¡!8gÎÏ4ñqñ3LKHLALLøc	ªï§6â0š·|ÙZñßlX#Uú³	ƒa“?-k@‚!_Ir™Æ,Ñ&<"î›GÊ¿2_CÓÜ«ŒUÌT�L1ƒáiÚý|È7]x05t²Ž£ß½.ãs<³V‹0Æ•»3Æ×€�L‚±‚EÅ0Ëø•0Â7[®Ì×2Ò†Ú^o8}=£ˆããyb¼2šº»‡ÑßÛ]&š®ý…'1à2Û‚Í -ôÕ³8¤Àý¥+‹°Ì¸ËƒƒƒefíìÖÕ­<dÁp1àHö/
­u}�Xáal¯Þ1Ž
=q´`Î5ŒÏiñ/ŒÊÁ›°’œqŸtíŠfüò—ßm]«>8WÓ |èã^îÐ=!@ÖÓŠ?.ÞU_ƒ?YÍ_>ïó2ƒÆ¶–4àÜt*üx04„ÍsZX„¤íí¤7ý§z˜ëÃ²bˆ�h„Á—Z·tÓ˜}†œÞ,ªuË!àƒ)ýz[>�7oþ¬ôàÉdÅüß?¯×J3O…ƒê>
¼7ÑL#ÁSƒ�#¯„<g‹,¿í^Ü)Æ3öç}r_›öœ-o<¨¯Üo
)ìíÚ–(aSƒ�G1iª† Z      >  Z    !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  #î<?xml version="1.0"?><Features nextValidID="17"><Feature recentID="15" name="SHLD2" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3677" maxFusedRunOn="3677"><Segment range="936-3647" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="16" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3677" maxFusedRunOn="3677" detectionMode="exactProteinMatch"><Segment range="3648-3674" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="14" name="lacI" directionality="1" translateFirstCodonAsMet="1" translationMW="38559.52" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6037-7119" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli. &lt;/i&gt;This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="13" name="AmpR" directionality="1" translationMW="31516.09" type="CDS" allowSegmentOverlaps="0" cleavageArrows="4164" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment name="signal sequence" range="4096-4164" color="#ccffcc" type="standard" translated="1"/><Segment range="4165-4956" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="12" name="GST" directionality="1" translationMW="25499.14" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3677" maxFusedRunOn="3677"><Segment range="258-911" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;glutathione &lt;i&gt;S&lt;/i&gt;-transferase from &lt;i&gt;Schistosoma japonicum&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="11" name="ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5127-5715" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="lacZÎ±" directionality="1" translationMW="19741.77" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="7242-81" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;LacZÎ± fragment of Î²-galactosidase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacZ&lt;/i&gt; fragment&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="9" name="AmpR promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3991-4095" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="lacIq promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5959-6036" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;In the &lt;i&gt;lacI&lt;sup&gt;q&lt;/sup&gt;&lt;/i&gt; allele, a single base change in the promoter boosts expression of the &lt;i&gt;lacI&lt;/i&gt; gene about 10-fold.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacI&lt;/i&gt; (mutant)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="lac promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-35" range="7168-7173" color="#ffffff" type="standard"/><Segment range="7174-7191" color="#ffffff" type="standard"/><Segment name="-10" range="7192-7198" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="tac promoter" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-35" range="183-188" color="#ffffff" type="standard"/><Segment range="189-204" color="#ffffff" type="standard"/><Segment name="-10" range="205-211" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;strong &lt;i&gt;E. coli&lt;/i&gt; promoter; hybrid between the &lt;i&gt;trp&lt;/i&gt; and &lt;i&gt;lac&lt;/i&gt;&amp;nbsp;UV5 promoters&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="CAP binding site" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7132-7153" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="thrombin site" directionality="1" translationMW="627.76" type="CDS" allowSegmentOverlaps="0" cleavageArrows="929" consecutiveTranslationNumbering="1" maxRunOn="3677" maxFusedRunOn="3677" detectionMode="exactProteinMatch"><Segment range="918-935" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;thrombin recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="lac operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="219-235" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="lac operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7206-7222" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="M13 rev" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7230-7246" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="7262-7278" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  B<Notes>
<UUID>df309827-8a3f-491e-8694-d1d088504390</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2016.10.14</Created>
<LastModified UTC="1:26:57">2021.12.20</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  0#     N   ®         ®r›7ßXëƒ-=°Sˆ&#BÉ[xÙk"ÎÔ3{ò¸bªŒñÛeÂ¸5f¸VŠim°ì™b Z!0eÖ­ÝtšØ¿æy
hi@ú#_ÞÇ®¨n…Y…j&1…e`ÅuT¨Z J¿[¯¦™¡‚¥iwK˜=¶r™“‡SuØ  ¢õQ24F­c¡A0ªiÂ*ÄZ6d•¡^íR†«¤ÔçáXáV+l©‹e]š•‚b_Ûáëb‰‡;¡Š*™ØÖ#àc+õZJŠ¥UÉ¡NoÝZIèVôÔ™“`ú�ƒSÜ¨ ƒo½VÁ¸+Ì�½…HU=‘Õ„>Qú¾=%æx´“ÃV)ÔV°þ�Z›•öáŽ9C„ÙvU‹_o±Ô´V÷õjÍ¶Tú4¬W»Ž½¨Õ…uk®Á'­¥½ÕŽµ�N8†f
gSºÑW—]™ÓZ¶’U„§Ôo±ÄM8ÓN=šXôØWÐ#Mm)|ÕÑ8
åE¾’¾ÑƒóÞA"›k„KõíÑÑ‰¨¸‚fÓj+S·ù5ÁÑÿß}ûRPH§¾÷ß½D=ÿïQ	×½ûÿ­€_W»WTÎk�]�g%»ñnï‹÷àƒQ›ï}ûµÞ¿öûåµDCi+Šø Ñ×ÖÛcD®MZ—«Û@GàXÑ`žW†­†wÕb‹øàÞ!l4÷ß½›î4å�Õ?®:�] Ž;ë-™Õ�I¯´¹†õtVKeØ™—i"~�]Z´¶ëŽôý-¿ÄÒa[ùØ5ùv}!Vº¥—ö�VDÔ·¸å®ÓëŽuÒÞ5§};æµÓâIŒ‰¥_DçGŽ¤ß�ßë gÝ=kWŠ×ô¾«á|ëV!â÷âŽïW›P}÷ßçõ]¸ã}Wöâ¾öq-m%>MQÓÔèýAF!TJaV(d€QTWF�zßšx¤]EFLPìšÕ)®ûSjVº˜]‰b{Ví´Ó.vÉUu¤E…aŒ
›µýÕ„Tc¨õT[–�¼ºU•`u.b¢eGy�M¨!K‹“QT‘žPê&5Õ^ë®ºÅmK;b”8DFoƒ…8u…€’²æi­¯Q”jg¨–�¯€fOšZE€Û«ÑRàÜk�“Eéø�(TŠÙV©­‘¥~f¨hl†f•¡áê¸Þ´`wq®Ry´H!ö÷FM�«Ia` oíN,btxSÚDÁ�ÏMôÆ.MÚê
ïÖ™£~Š9ÅÐgwJ"("šê¡X €uûêè7W}¤WuaB›^]»­Ú÷ÝNW´¾ë†é¦•ê<AüQ³¤5á…eS¶öª•u¡ë½!øGM.:ÙÚùVÝß×FvÕªâ†øei�!†\AEX•‡{µ�‚eVM¾ck˜ZŸ¯*¨aÖfZoù¡fv–·PíM,Q†ÕwC´F•[1¹&b§öqÉôiÎ”ÃÍ²n>ÌÊ°†!WîØÓ¿p·N‹‰LðGSòÐ3-0"ƒ,4öÒÜ“0H™k¡Gì†sæªd)bØe–
ºG-7ªÀˆÌþj*j®Vvµf&ó¶Ö_á•Žµ–hUAD˜I­v¯"Ïé¯ê¨Ãj�¯Š¨íýÎT•F8Q´ #q )Û�»]0”o¡«?Å*½a„Þ©R¸æ Ì&›ò(sÀÔM{<ÙÞk´Xz
{}-ÑTÛ§‡IDZcHãÙ}„†]ã^ž¸uÆÁÅåóª£uØ m‰{>†Ê|……=ŸWL‡}Æ>4ÖuÊ3Wš^^z{“¯Ã]idxÁ•(Ð}ëŒì?¯
š8enð„È³	¶�7€=%?¿&É)¹Üp´5æ‘Ñ³„d#íá¥6‚^-R§¶fz‘¡©W­ª–¥PnBoª†[¶U¿¥ÈJ¬¯xL´ÉFÕë¡êªã2[Ž:5uU}óŸL ¨¨ðmDç×º«Å	x:ÓDÍõg‹ë’$òJCßzÔ^âMóçgìî9oŠ} ã�H1”².Ÿ�íG)ðeÓ…À²7tý:ÅÇç|^Ÿ�2˜EL÷:üPPÁÐ›ÂSÁt3q:ô W>	º™/‹¬÷î„Ë¨†;- æW=5Ì>ªp¯ƒêŽª;v+«ã)¿á® eòióê†|ìóO4Ä´„ÄÁDÏ†0š®úsn#	«wÍ•¯öÅ‚5_«0˜63ðQÖ´!bô—)œbÍcÂ.é´|«ó …ô==Ê¸Å\ÀUHÃ0N�¯×Ìƒu×ƒWK(á Ê=ûÒàþ73Ë5h³i[³<mxôÈ ‹$\S±O‰S#uºìÍs- 8m¥æó�‡ÐcÚ8�N>7–+Ã)«¡‹¸}ýµÒiªïØQÂs M¸,ÚßM[3ŠLÚR¸»ËŒ¸886VnÞÍmZÓÆLC�bðª×WØÕŽ6Êýãà£×Fà3XÌöŸð8Ì Œ»	 ÇñGNØ¦oÏ)}öÕÐj³ã€5}2ÎŽ5îíÐÂm8£òíà…QU÷ø3õœÕóîÿ3(<kicNÇB¯Ç�SHL×5¥ˆJNÞÚCÐêw©Ž¼;&(ˆˆLu«wA]9‡ØiÁ­àòÊ§\²Wø2Ÿ×¥³èÓvÿêÏN–LQÍñ³úýt£4øX1`Î£à«Ã}Â<881ºøCÆx²ËþÕàÑ�Âœc?nw×%ù¿iÂÖóÊŠýÁñÀ¢žÎÝ©b†89ÐPôsš¨`Ê
      ä      ­  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  $<?xml version="1.0"?><Features nextValidID="17"><Feature recentID="16" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4355" maxFusedRunOn="4355" detectionMode="exactProteinMatch"><Segment range="4326-4352" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="15" name="LATS1" directionality="1" translationMW="126873.19" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4355" maxFusedRunOn="4355"><Segment range="936-4325" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="14" name="lacI" directionality="1" translateFirstCodonAsMet="1" translationMW="38559.52" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6715-7797" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli. &lt;/i&gt;This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="13" name="AmpR" directionality="1" translationMW="31516.09" type="CDS" allowSegmentOverlaps="0" cleavageArrows="4842" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment name="signal sequence" range="4774-4842" color="#ccffcc" type="standard" translated="1"/><Segment range="4843-5634" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="12" name="GST" directionality="1" translationMW="25499.14" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4355" maxFusedRunOn="4355"><Segment range="258-911" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;glutathione &lt;i&gt;S&lt;/i&gt;-transferase from &lt;i&gt;Schistosoma japonicum&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="11" name="ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5805-6393" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="lacZÎ±" directionality="1" translationMW="19741.77" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="7920-81" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;LacZÎ± fragment of Î²-galactosidase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacZ&lt;/i&gt; fragment&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="9" name="AmpR promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4669-4773" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="lacIq promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6637-6714" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;In the &lt;i&gt;lacI&lt;sup&gt;q&lt;/sup&gt;&lt;/i&gt; allele, a single base change in the promoter boosts expression of the &lt;i&gt;lacI&lt;/i&gt; gene about 10-fold.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacI&lt;/i&gt; (mutant)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="lac promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-35" range="7846-7851" color="#ffffff" type="standard"/><Segment range="7852-7869" color="#ffffff" type="standard"/><Segment name="-10" range="7870-7876" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="tac promoter" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-35" range="183-188" color="#ffffff" type="standard"/><Segment range="189-204" color="#ffffff" type="standard"/><Segment name="-10" range="205-211" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;strong &lt;i&gt;E. coli&lt;/i&gt; promoter; hybrid between the &lt;i&gt;trp&lt;/i&gt; and &lt;i&gt;lac&lt;/i&gt;&amp;nbsp;UV5 promoters&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="CAP binding site" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7810-7831" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="thrombin site" directionality="1" translationMW="627.76" type="CDS" allowSegmentOverlaps="0" cleavageArrows="929" consecutiveTranslationNumbering="1" maxRunOn="4355" maxFusedRunOn="4355" detectionMode="exactProteinMatch"><Segment range="918-935" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;thrombin recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="lac operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="219-235" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="lac operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7884-7900" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="M13 rev" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7908-7924" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="7940-7956" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  B<Notes>
<UUID>df309827-8a3f-491e-8694-d1d088504390</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2016.10.14</Created>
<LastModified UTC="1:26:44">2021.12.20</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
   ±<StrandColors><TopStrand><ColorRange range="2380..2380" colors="purple"/></TopStrand><BottomStrand><ColorRange range="2380..2380" colors="purple"/></BottomStrand></StrandColors>  /Á     N   ­         ­r›7ßXëƒ-=°Sˆ&#BÉ[xÙk"ÎÔ3{ò¸bªŒñÛeÂ¸5f¸VŠim°ì™b Z!0eÖ­ÝtšØ¿æy
hi@ú#_ÞÇ®¨n…Y…j&1…e`ÅuT¨Z J¿[¯¦™¡‚¥iwK˜=¶r™“‡SuØ  ¢õQ24F­c¡A0ªiÂ*ÄZ6d•¡^íR†«¤ÔçáXáV+l©‹e]š•‚b_Ûáëb‰‡;¡Š*™ØÖ#àc+õZJŠ¥UÉ¡NoÝZIèVôÔ™“`ú�ƒSÜ¨ ƒo½VÁ¸+Ì�½…HU=‘Õ„>Qú¾=%æx´“ÃV)ÔV°þ�Z›•öáŽ9C„ÙvU‹_o±Ô´V÷õjÍ¶Tú4¬W»Ž½¨Õ…uk®Á'­¥½ÕŽµ�N8†f
gSºÑW—]™ÓZ¶’U„§Ôo±ÄM8ÓN=šXôØWÐ#Mm)|ÕÑ8
åE¾’¾ÑƒóÞA"›k„KõíÑÑ‰¨¸‚fÓj+S·ù5ÁÑÿß}ûRPH§¾÷ß½D=ÿïQ	×½ûÿ­€_W»WTÎk�]�g%»ñnï‹÷àƒQ›ï}ûµÞ¿öûåµDCi+Šø Ñ×ÖÛcD®MZ—«Û@GàXÑ`žW†­†wÕb‹øàÞ!l4÷ß½›î4å�Õ?®:�] Ž;ë-™Õ�I¯´¹†õtVKeØ™—i"~�]Z´¶ëŽôý-¿ÄÒa[ùØ5ùv}!Vº¥—ö�VDÔ·¸å®ÓëŽuÒÞ5§};æµÓâIŒ‰¥_DçGŽ¤ß�ßë gÝ=kWŠ×ô¾«á|ëV!â÷âŽïW›P}÷ßçõ]¸ã}Wöâ¾öq-m%>MQÓÔèýAF!TJaV(d€QTWF�zßšx¤]EFLPìšÕ)®ûSjVº˜]‰b{Ví´Ó.vÉUu¤E…aŒ
›µýÕ„Tc¨õT[–�¼ºU•`u.b¢eGy�M¨!K‹“QT‘žPê&5Õ^ë®ºÅmK;b”8DFoƒ…8u…€’²æi­¯Q”jg¨–�¯€fOšZE€Û«ÑRàÜk�“Eéø�(TŠÙV©­‘¥~f¨hl†f•¡áê¸Þ´`wq®Ry´H!ö÷FM�«Ia` oíN,btxSÚDÁ�ÏMôÆ.MÚê
ïÖ™£~Š9ÅÐgwJ"("šê¡X €uûêè7W}¤WuaB›^]»­Ú÷ÝNW´¾ë†é¦•ê<AüQ³¤5á…eS¶öª•u¡ë½!øGM.:ÙÚùVÝß×FvÕªâ†øei�!†\AEX•‡{µ�‚eVM¾ck˜ZŸ¯*¨aÖfZoù¡fv–·PíM,Q†ÕwC´F•[1¹&b§öqÉôiÎ“6Èxxq¸û3*Âp…_¸wcNüÂÝ:.%0cÀMOË@Ì´ÀŠ°ÓØKrLdÁ"e®…T²Ïš©�¥‹a–X*é´ Þ« V#0_ù¨©ª¹YÚÕ˜›ÎØGY†V:ÖY¡Ua&µÚ¼‹?¦¿ª£ª>¾*£·ôW8!RUáFÒ€�Ä€§l^ítÂQ¾„J¬ÿ\ªõ†z¥Jã˜ƒ0šoÈ¡ÏQ5ì,ógy®Ñaè(Iíô·ESnœ6%i�#�eöwŒ=zzá×—Îª�×`¶%ìú(ðNö}\q2÷øÓX5×(Í^ix1yéîN¿u¥‘à`CT£A÷®3°þ¼88*há•¸Â .Ì&Ú4Þ ô`”þü›$¦çqÀÐ×˜\VGFÎ��·†”Ú	x´}JžÙ™êF†¥^¶ªZ•A¹Q¾ªnØ}Vþ—!*²½á2Ñÿ$UT®„oª«ŒÉn8èÕÕU÷Î}0‚¢£ÁµŸ^ê¯%àëM4gÕž/®H“É)}ëQ{‰7Ï�Ÿ³¸å¾)ôƒŽ= ÆRÈº|
µd§Á”3L, 
ÈÝÓôè�ñxF~tÈNa3Üëñ@ECBo	L4ÐDÍÄëÐ\ø&êd¾,
³ß¸z,j¢ì´ 0O˜\ô×0ú©Â¾ª8>¨íØ®¯Œ¦ÿ†¸�—É§Ï¨HNó³Í<\|LÓÒS>Âj»éÍ¸Œ&­ß6V¼wÛÕ~¬Â`ØdÏÁDKZÐ …ˆWÒ\¦q‹4I�»¦Ñò¯Ì‚Ðô÷*ãsU S`Á8Zv¿_2×^],£†ƒ(÷ïKƒøÜÏ,Õ¢Ì1¥nÌñµà#Ó ‚,`‘qL2Å>%L0�Öë³5Ì´€á¶—›ÎA�hâ8øÞX¯¦®†.áôwö×I¦«¿aG	Ìx�6à³h}5lÎ)0?iJâì3.2àààÙY»{5µkO0\x=‹Â«]_cV8X@Û+÷Œc‚�\m3€Íc3Ú|KÀã2‚0fì$€ggÅ;b™¿<¥÷ÛWAªÏŽ ÕôÈ:8×»´CP5´â�Ë·‚EWßàÏÖsWÏ»üÌ ñ­¥�87
¿L!3\Ö–!);{iÿC©Þ¦:ðì˜¢ Z!0eÖ­Ýtæa§·ƒË*�rÈx_àÊ^–Ï£MÛÿ«=82Y1D?7ÆÏëõÒŒÓá`Åƒ:�‚¯ôSðTààHÆëáâË/ûWƒF7
qŒý¹ß\—æý§[Ï*+÷[ÇŠ{;v¥ŠTàçACÑÌZj¡ƒ(      ä      ¬  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  $<?xml version="1.0"?><Features nextValidID="17"><Feature recentID="16" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4355" maxFusedRunOn="4355" detectionMode="exactProteinMatch"><Segment range="4326-4352" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="15" name="LATS1" directionality="1" translationMW="126873.19" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4355" maxFusedRunOn="4355"><Segment range="936-4325" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="14" name="lacI" directionality="1" translateFirstCodonAsMet="1" translationMW="38559.52" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6714-7796" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli. &lt;/i&gt;This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="13" name="AmpR" directionality="1" translationMW="31516.09" type="CDS" allowSegmentOverlaps="0" cleavageArrows="4841" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment name="signal sequence" range="4773-4841" color="#ccffcc" type="standard" translated="1"/><Segment range="4842-5633" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="12" name="GST" directionality="1" translationMW="25499.14" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4355" maxFusedRunOn="4355"><Segment range="258-911" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;glutathione &lt;i&gt;S&lt;/i&gt;-transferase from &lt;i&gt;Schistosoma japonicum&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="11" name="ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5804-6392" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="lacZÎ±" directionality="1" translationMW="19741.77" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="7919-81" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;LacZÎ± fragment of Î²-galactosidase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacZ&lt;/i&gt; fragment&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="9" name="AmpR promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4668-4772" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="lacIq promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6636-6713" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;In the &lt;i&gt;lacI&lt;sup&gt;q&lt;/sup&gt;&lt;/i&gt; allele, a single base change in the promoter boosts expression of the &lt;i&gt;lacI&lt;/i&gt; gene about 10-fold.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacI&lt;/i&gt; (mutant)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="lac promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-35" range="7845-7850" color="#ffffff" type="standard"/><Segment range="7851-7868" color="#ffffff" type="standard"/><Segment name="-10" range="7869-7875" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="tac promoter" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-35" range="183-188" color="#ffffff" type="standard"/><Segment range="189-204" color="#ffffff" type="standard"/><Segment name="-10" range="205-211" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;strong &lt;i&gt;E. coli&lt;/i&gt; promoter; hybrid between the &lt;i&gt;trp&lt;/i&gt; and &lt;i&gt;lac&lt;/i&gt;&amp;nbsp;UV5 promoters&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="CAP binding site" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7809-7830" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="thrombin site" directionality="1" translationMW="627.76" type="CDS" allowSegmentOverlaps="0" cleavageArrows="929" consecutiveTranslationNumbering="1" maxRunOn="4355" maxFusedRunOn="4355" detectionMode="exactProteinMatch"><Segment range="918-935" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;thrombin recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="lac operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="219-235" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="lac operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7883-7899" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="M13 rev" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7907-7923" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="7939-7955" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  –<Notes>
<UUID>df309827-8a3f-491e-8694-d1d088504390</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pGEX-4T-2</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="16:0:0">2016.10.14</Created>
<LastModified UTC="1:20:36">2021.12.20</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  /À     M   «         «r›7ßXëƒ-=°Sˆ&#BÉ[xÙk"ÎÔ3{ò¸bªŒñÛeÂ¸5f¸VŠim°ì™b Z!0eÖ­ÝtšØ¿æy
hi@ú#_ÞÇ®¨n…Y…j&1…e`ÅuT¨Z J¿[¯¦™¡‚¥iwK˜=¶r™“‡SuØ  ¢õQ24F­c¡A0ªiÂ*ÄZ6d•¡^íR†«¤ÔçáXáV+l©‹e]š•‚b_Ûáëb‰‡;¡Š*™ØÖ#àc+õZJŠ¥UÉ¡NoÝZIèVôÔ™“`ú�ƒSÜ¨ ƒo½VÁ¸+Ì�½…HU=‘Õ„>Qú¾=%æx´“ÃV)ÔV°þ�Z›•öáŽ9C„ÙvU‹_o±Ô´V÷õjÍ¶Tú4¬W»Ž½¨Õ…uk®Á'­¥½ÕŽµ�N8†f
gSºÑW—]™ÓZ¶’U„§Ôo±ÄM8ÓN=šXôØWÐ#Mm)|ÕÑ8
åE¾’¾ÑƒóÞA"›k„KõíÑÑ‰¨¸‚fÓj+S·ù5ÁÑÿß}ûRPH§¾÷ß½D=ÿïQ	×½ûÿ­€_W»WTÎk�]�g%»ñnï‹÷àƒQ›ï}ûµÞ¿öûåµDCi+Šø Ñ×ÖÛcD®MZ—«Û@GàXÑ`žW†­†wÕb‹øàÞ!l4÷ß½›î4å�Õ?®:�] Ž;ë-™Õ�I¯´¹†õtVKeØ™—i"~�]Z´¶ëŽôý-¿ÄÒa[ùØ5ùv}!Vº¥—ö�VDÔ·¸å®ÓëŽuÒÞ5§};æµÓâIŒ‰¥_DçGŽ¤ß�ßë gÝ=kWŠ×ô¾«á|ëV!â÷âŽïW›P}÷ßçõ]¸ã}Wöâ¾öq-m%>MQÓÔèýAF!TJaV(d€QTWF�zßšx¤]EFLPìšÕ)®ûSjVº˜]‰b{Ví´Ó.vÉUu¤E…aŒ
›µýÕ„Tc¨õT[–�¼ºU•`u.b¢eGy�M¨!K‹“QT‘žPê&5Õ^ë®ºÅmK;b”8DFoƒ…8u…€’²æi­¯Q”jg¨–�¯€fOšZE€Û«ÑRàÜk�“Eéø�(TŠÙV©­‘¥~f¨hl†f•¡áê¸Þ´`wq®Ry´H!ö÷FM�«Ia` oíN,btxSÚDÁ�ÏMôÆ.MÚê
ïÖ™£~Š9ÅÐgwJ"("šê¡X €uûêè7W}¤WuaB›^]»­Ú÷ÝNW´¾ë†é¦•ê<AüQ³¤5á…eS¶öª•u¡ë½!øGM.:ÙÚùVÝß×FvÕªâ†øei�!†\AEX•‡{µ�‚eVM¾ck˜ZŸ¯*¨aÖfZoù¡fv–·PíM,Q†ÕwC´F•[1¹&b§öqÉôiÎ0ól‡‡‡�³2¬!‡Uû‡v4ïÁ-Ó¢âS<ÑÔü´ËL À‹ Í=‡ô·$ÆL&ZèQÅ@û!œùª™
X¶e‚®‘ËBê°b3ÿšŠš«•�­Y‰¼í„u—øec­ešPQ&k]«È³úkúª0Ú£ëâª;Es‚%QŽm(ÜH
vÅàn×L%èDªÏñEÊ¯Xa7ªT®9ˆ3	¦üŠð5^ÂÏ6wší‚„žßKtU6éÁƒaÒQ˜Ò8ö_a!—xÃ×§®q°AñA9|ê¨Ýv b^Ï¡²€_áaOgÕÇ!ßq��5ƒ]rŒÕæ—ƒžžäëð×ZY0eJ4zã;ëÃƒ‚¦Ž[�<!2ìÂm£MàF	OïÉ²Jnw-y�…ÅdtláûxiM —‹GÔ©í™ž¤hjUëj¥©T�…ê¡–í‡Õoér«+Þ-òEQµAºèFúª¸Ì–ãŽ�]U_|çÓ**<Q9õîªñB^´Ñ3F}Yâúä‰<’�÷Þµ¸“|ùÙû;Ž[âŸH8ãÒe,‹§À {QÆJ|C4ÂÀáp ¬�Ý?N�1qùß„gçL„æS=Î¿T0t&ð”Ã@°]LÜ@N½ Ï‚n¦KâÀ«=û‡¡2Æª!ŽË@ù�UÏMsªœ+àú£ƒêŽÝŠêøÊoøkˆ|š|ú„„áŸ;<ÓÅÇÄÍ1-!10Q3áŒ&«¾œÛˆÂjÝóekÇ}±`�WêÌ&†LüDµ­X…}%Êg³D˜ð‹ºm*üÈ!}Or®1W0R0Æ…§kõó ÝuàÀÕÒÊ8h2�~ô¸?�ÌòÍZ,ÃVìÏ^=2"Æ	Ã,SâTÃÝn»3\ËHiy¼àaôöŽ S��åŠðÊjèbîGmtšj»öpœÇ€Èn6€·ÓVÌâ“ö”®.Ã2ã.•›·³[V´ñ“ÀPÇ�#Ø¼*µÕö5c…„²¿xÆ8(õÆÑƒ8Ö3=§Ä¼3(#nÂHqÆ|QÓ¶)›óÊ_}µt¬øà_L�ó£�{»@ô0…[N(ü»x!TU}þýg5|û¿ÌÊÚXÓƒqÐ«ñàTÀÒ5Íib“·¶�ßô:�êc¯ÉŠ"¢]jÝÐWNaöpkx<²©×,‡€Uþ§õélú4Ý¿ú³Óƒ%“Có|lþ¿](Í>X3¨ø*ðßE0�NŒn¾ñž,²ÿµx4cp§ÏÛ�õÉ~oÚpµ¼ò¢¿pE¼p(§³·jX¡…Nt=Å¦ª2�      ä      ª  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  $<?xml version="1.0"?><Features nextValidID="17"><Feature recentID="16" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4367" maxFusedRunOn="4367" detectionMode="exactProteinMatch"><Segment range="4326-4352" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="15" name="LATS1" directionality="1" translationMW="126873.19" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4367" maxFusedRunOn="4367"><Segment range="936-4325" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="14" name="lacI" directionality="1" translateFirstCodonAsMet="1" translationMW="38559.52" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6712-7794" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli. &lt;/i&gt;This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="13" name="AmpR" directionality="1" translationMW="31516.09" type="CDS" allowSegmentOverlaps="0" cleavageArrows="4839" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment name="signal sequence" range="4771-4839" color="#ccffcc" type="standard" translated="1"/><Segment range="4840-5631" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="12" name="GST" directionality="1" translationMW="25499.14" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4367" maxFusedRunOn="4367"><Segment range="258-911" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;glutathione &lt;i&gt;S&lt;/i&gt;-transferase from &lt;i&gt;Schistosoma japonicum&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="11" name="ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5802-6390" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="lacZÎ±" directionality="1" translationMW="19741.77" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="7917-81" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;LacZÎ± fragment of Î²-galactosidase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacZ&lt;/i&gt; fragment&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="9" name="AmpR promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4666-4770" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="lacIq promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6634-6711" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;In the &lt;i&gt;lacI&lt;sup&gt;q&lt;/sup&gt;&lt;/i&gt; allele, a single base change in the promoter boosts expression of the &lt;i&gt;lacI&lt;/i&gt; gene about 10-fold.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacI&lt;/i&gt; (mutant)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="lac promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-35" range="7843-7848" color="#ffffff" type="standard"/><Segment range="7849-7866" color="#ffffff" type="standard"/><Segment name="-10" range="7867-7873" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="tac promoter" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-35" range="183-188" color="#ffffff" type="standard"/><Segment range="189-204" color="#ffffff" type="standard"/><Segment name="-10" range="205-211" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;strong &lt;i&gt;E. coli&lt;/i&gt; promoter; hybrid between the &lt;i&gt;trp&lt;/i&gt; and &lt;i&gt;lac&lt;/i&gt;&amp;nbsp;UV5 promoters&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="CAP binding site" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7807-7828" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="thrombin site" directionality="1" translationMW="627.76" type="CDS" allowSegmentOverlaps="0" cleavageArrows="929" consecutiveTranslationNumbering="1" maxRunOn="4367" maxFusedRunOn="4367" detectionMode="exactProteinMatch"><Segment range="918-935" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;thrombin recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="lac operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="219-235" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="lac operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7881-7897" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="M13 rev" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7905-7921" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="7937-7953" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  –<Notes>
<UUID>df309827-8a3f-491e-8694-d1d088504390</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pGEX-4T-2</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="16:0:0">2016.10.14</Created>
<LastModified UTC="1:20:31">2021.12.20</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  -²     >   �         �r›7ßXëƒ-=°Sˆ&#BÉ[xÙk"ÎÔ3{ò¸bªŒñÛeÂ¸5f¸VŠim°ì™b Z!0eÖ­ÝtšØ¿æy
hi@ú#_ÞÇ®¨n…Y…j&1…e`ÅuT¨Z J¿[¯¦™¡‚¥iwK˜=¶r™“‡SuØ  ¢õQ24F­c¡A0ªiÂ*ÄZ6d•¡^íR†«¤ÔçáXáV+l©‹e]š•‚b_Ûáëb‰‡;¡Š*™ØÖ#àc+õZJŠ¥UÉ¡NoÝZIèVôÔ™“`ú�ƒSÜ¨ ƒo½VÁ¸+Ì�½…HU=‘Õ„>Qú¾=%æx´“ÃV)ÔV°þ�Z›•öáŽ9C„ÙvU‹_o±Ô´V÷õjÍ¶Tú4¬W»Ž½¨Õ…uk®Á'­¥½ÕŽµ�N8†f
gSºÑW—]™ÓZ¶’U„§Ôo±ÄM8ÓN=šXôØWÐ#Mm)|ÕÑ8
åE¾’¾ÑƒóÞA"›k„KõíÑÑ‰¨¸‚fÓj+S·ù5ÁÑÿß}ûRPH§¾÷ß½D=ÿïQ	×½ûÿ­€_W»WTÎk�]�g%»ñnï‹÷àƒQ›ï}ûµÞ¿öûåµDCi+Šø Ñ×ÖÛcD®MZ—«Û@GàXÑ`žW†­†wÕb‹øàÞ!l4÷ß½›î4å�Õ?®:�] Ž;ë-™Õ�I¯´¹†õtVKeØ™—i"~�]Z´¶ëŽôý-¿ÄÒa[ùØ5ùv}!Vº¥—ö�VDÔ·¸å®ÓëŽuÒÞ5§};æµÓâIŒ‰¥_DçGŽ¤ß�ßë gÝ=kWŠ×ô¾«á|ëV!â÷âŽïW›P}÷ßçõ]¸ã}Wöâ¾öq-m%>MQÓÔèýAF!TJaV(d€QTWF�zßšx¤]EFLPìšÕ)®ûSjVº˜]‰b{Ví´Ó.vÉUu¤E…aŒ
›µýÕ„Tc¨õT[–�¼ºU•`u.b¢eGy�M¨!K‹“QT‘žPê&5Õ^ë®ºÅmK;b”8DFoƒ…8u…€’²æi­¯Q”jg¨–�¯€fOšZE€Û«ÑRàÜk�“Eéø�(TŠÙV©­‘¥~f¨hl†f•¡áê¸Þ´`wq®Ry´H!ö÷FM�«Ia` oíN,btxSÚDÁ�ÏMôÆ.MÚê
ïÖ™£~Š9ÅÐgwJ"("šê¡X €uûêè7W}¤WuaB›^]»­Ú÷ÝNW´¾ë†é¦•ê<AüQ³¤5á…eS¶öª•u¡ë½!øGM.:ÙÚùVÝß×FvÕªâ†øei�!†\AEX•‡{µ�‚eVM¾ck˜ZŸ¯*¨aÖfZoù¡fv–·PíM,Q†ÕwC´F•[1¹&bŒ<Û!ááÆãìÌ«aÂ~áÝ�;ðGtè¸”Á�4u?-2Ó(0"À3Oaý-É1“‰–ºqP>Èg>j¦B–-†Y`«¤rÐƒz¬XŒÁæ¢¦ªågkVbo;aeþXëYf…TI„š×jò,þšþªŒ6¨úøªŽßÑ\à…ITc…J7�±xµÓ	Fú*³üQr«ÖMê•+ŽbÂi¿"‡<D×°³Í�æ»E‡ ¡'·ÒÝMºp`Øt”E¦4Ž=—ØHeÞ0õéë‡\l|PN_:ª7]€Ø—³èl Á8XSÙõqÄÈwÜcãM`×\£5y¥àÅç§¹:ü5Ö–G��R�Þ¸ÎÃúðàà©£†VàOL€»0›hÓxÑ‚Sûòl’›�Ç KC^`aqY8FB>ÞSh%âÑõ*{fg©•zÚ©jUä!Fú¨e»aõ[ú\„ªÊ÷„ËGü‘TmPnº¾ª®3%¸ã£WUWß9ôÂ
Š�ÔN}{ª¼P—ƒ­4LÑŸVx¾¹"O$¤=÷­Eî$ß>v~Îã–ø§Ò8ôƒK"éð(Ôq’ŸPÍ0°8\ +#wOÓ L\~wÅáùÓ!9„TÏs¯Å	¼%0Ð,A7¯@sà›©’ø°*Ï~áèL±ªˆc²Ð Á>`UsÓ\Ãê§
ø>¨àú£·bº¾2›þâ_&Ÿ>¡!8gÎÏ4ñqñ3LKHLALLøc	ªï§6â0š·|ÙZñßlX#Uú³	ƒa“?-k@‚!_Ir™Æ,Ñ&<"î›GÊ¿2_CÓÜ«ŒUÌT�L1ƒáiÚý|È7]x05t²Ž£ß½.ãs<³V‹0Æ•»3Æ×€�L‚±‚EÅ0Ëø•0Â7[®Ì×2Ò†Ú^o8}=£ˆããyb¼2šº»‡ÑßÛ]&š®ý…'1à2Û‚Í -ôÕ³8¤Àý¥+‹°Ì¸ËƒƒƒefíìÖÕ­<dÁp1àHö/
­u}�Xáal¯Þ1Ž
=q´`Î5ŒÏiñ/ŒÊÁ›°’œqŸtíŠfüò—ßm]«>8WÓ |èã^îÐ=!@ÖÓŠ?.ÞU_ƒ?YÍ_>ïó2ƒÆ¶–4àÜt*üx04„ÍsZX„¤íí¤7ý§z˜ëÃ²bˆ�h„Á—Z·tÓ˜}†œÞ,ªuË!àƒ)ýz[>�7oþ¬ôàÉdÅüß?¯×J3O…ƒê>
¼7ÑL#ÁSƒ�#¯„<g‹,¿í^Ü)Æ3öç}r_›öœ-o<¨¯Üo
)ìíÚ–(aSƒ�G1iª† Z       �  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  "	<?xml version="1.0"?><Features nextValidID="16"><Feature recentID="15" name="LATS1" directionality="1" translationMW="126873.19" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4340" maxFusedRunOn="4340"><Segment range="936-4325" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="14" name="lacI" directionality="1" translateFirstCodonAsMet="1" translationMW="38559.52" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6685-7767" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli. &lt;/i&gt;This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="13" name="AmpR" directionality="1" translationMW="31516.09" type="CDS" allowSegmentOverlaps="0" cleavageArrows="4812" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment name="signal sequence" range="4744-4812" color="#ccffcc" type="standard" translated="1"/><Segment range="4813-5604" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="12" name="GST" directionality="1" translationMW="25499.14" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4340" maxFusedRunOn="4340"><Segment range="258-911" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;glutathione &lt;i&gt;S&lt;/i&gt;-transferase from &lt;i&gt;Schistosoma japonicum&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="11" name="ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5775-6363" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="lacZÎ±" directionality="1" translationMW="19741.77" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="7890-81" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;LacZÎ± fragment of Î²-galactosidase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacZ&lt;/i&gt; fragment&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="9" name="AmpR promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4639-4743" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="lacIq promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6607-6684" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;In the &lt;i&gt;lacI&lt;sup&gt;q&lt;/sup&gt;&lt;/i&gt; allele, a single base change in the promoter boosts expression of the &lt;i&gt;lacI&lt;/i&gt; gene about 10-fold.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacI&lt;/i&gt; (mutant)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="lac promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-35" range="7816-7821" color="#ffffff" type="standard"/><Segment range="7822-7839" color="#ffffff" type="standard"/><Segment name="-10" range="7840-7846" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="tac promoter" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-35" range="183-188" color="#ffffff" type="standard"/><Segment range="189-204" color="#ffffff" type="standard"/><Segment name="-10" range="205-211" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;strong &lt;i&gt;E. coli&lt;/i&gt; promoter; hybrid between the &lt;i&gt;trp&lt;/i&gt; and &lt;i&gt;lac&lt;/i&gt;&amp;nbsp;UV5 promoters&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="CAP binding site" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7780-7801" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="thrombin site" directionality="1" translationMW="627.76" type="CDS" allowSegmentOverlaps="0" cleavageArrows="929" consecutiveTranslationNumbering="1" maxRunOn="4340" maxFusedRunOn="4340" detectionMode="exactProteinMatch"><Segment range="918-935" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;thrombin recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="lac operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="219-235" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="lac operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7854-7870" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="M13 rev" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7878-7894" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="7910-7926" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  –<Notes>
<UUID>df309827-8a3f-491e-8694-d1d088504390</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pGEX-4T-2</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="16:0:0">2016.10.14</Created>
<LastModified UTC="1:20:27">2021.12.20</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  -†     ?   “         “r›7ßXëƒ-=°Sˆ&#BÉ[xÙk"ÎÔ3{ò¸bªŒñÛeÂ¸5f¸VŠim°ì™b Z!0eÖ­ÝtšØ¿æy
hi@ú#_ÞÇ®¨n…Y…j&1…e`ÅuT¨Z J¿[¯¦™¡‚¥iwK˜=¶r™“‡SuØ  ¢õQ24F­c¡A0ªiÂ*ÄZ6d•¡^íR†«¤ÔçáXáV+l©‹e]š•‚b_Ûáëb‰‡;¡Š*™ØÖ#àc+õZJŠ¥UÉ¡NoÝZIèVôÔ™“`ú�ƒSÜ¨ ƒo½VÁ¸+Ì�½…HU=‘Õ„>Qú¾=%æx´“ÃV)ÔV°þ�Z›•öáŽ9C„ÙvU‹_o±Ô´V÷õjÍ¶Tú4¬W»Ž½¨Õ…uk®Á'­¥½ÕŽµ�N8†f
gSºÑW—]™ÓZ¶’U„§Ôo±ÄM8ÓN=šXôØWÐ#Mm)|ÕÑ8
åE¾’¾ÑƒóÞA"›k„KõíÑÑ‰¨¸‚fÓj+S·ù5ÁÑÿß}ûRPH§¾÷ß½D=ÿïQ	×½ûÿ­€_W»WTÎk�]�g%»ñnï‹÷àƒQ›ï}ûµÞ¿öûåµDCi+Šø Ñ×ÖÛcD®MZ—«Û@GàXÑ`žW†­†wÕb‹øàÞ!l4÷ß½›î4å�Õ?®:�] Ž;ë-™Õ�I¯´¹†õtVKeØ™—i"~�]Z´¶ëŽôý-¿ÄÒa[ùØ5ùv}!Vº¥—ö�VDÔ·¸å®ÓëŽuÒÞ5§};æµÓâIŒ‰¥_DçGŽ¤ß�ßë gÝ=kWŠ×ô¾«á|ëV!â÷âŽïW›P}÷ßçõ]¸ã}Wöâ¾öq-m%>MQÓÔèýAF!TJaV(d€QTWF�zßšx¤]EFLPìšÕ)®ûSjVº˜]‰b{Ví´Ó.vÉUu¤E…aŒ
›µýÕ„Tc¨õT[–�¼ºU•`u.b¢eGy�M¨!K‹“QT‘žPê&5Õ^ë®ºÅmK;b”8DFoƒ…8u…€’²æi­¯Q”jg¨–�¯€fOšZE€Û«ÑRàÜk�“Eéø�(TŠÙV©­‘¥~f¨hl†f•¡áê¸Þ´`wq®Ry´H!ö÷FM�«Ia` oíN,btxSÚDÁ�ÏMôÆ.MÚê
ïÖ™£~Š9ÅÐgwJ"("šê¡X €uûêè7W}¤WuaB›^]»­Ú÷ÝNW´¾ë†é¦•ê<AüQ³¤5á…eS¶öª•u¡ë½!øGM.:ÙÚùVÝß×FvÕªâ†øei�!†\AEX•‡{µ�‚eVM¾ck˜ZŸ¯*¨aÖfZoù¡fv–·PíM,Q†ÕwC´F•[1¹&b¥0ól‡‡‡�³2¬!‡Uû‡v4ïÁ-Ó¢âS<ÑÔü´ËL À‹ Í=‡ô·$ÆL&ZèQÅ@û!œùª™
X¶e‚®‘ËBê°b3ÿšŠš«•�­Y‰¼í„u—øec­ešPQ&k]«È³úkúª0Ú£ëâª;Es‚%QŽm(ÜH
vÅàn×L%èDªÏñEÊ¯Xa7ªT®9ˆ3	¦üŠð5^ÂÏ6wší‚„žßKtU6éÁƒaÒQ˜Ò8ö_a!—xÃ×§®q°AñA9|ê¨Ýv b^Ï¡²€_áaOgÕÇ!ßq��5ƒ]rŒÕæ—ƒžžäëð×ZY0eJ4zã;ëÃƒ‚¦Ž[�<!2ìÂm£MàF	OïÉ²Jnw-y�…ÅdtláûxiM —‹GÔ©í™ž¤hjUëj¥©T�…ê¡–í‡Õoér«+Þ-òEQµAºèFúª¸Ì–ãŽ�]U_|çÓ**<Q9õîªñB^´Ñ3F}Yâúä‰<’�÷Þµ¸“|ùÙû;Ž[âŸH8ãÒe,‹§À {QÆJ|C4ÂÀáp ¬�Ý?N�1qùß„gçL„æS=Î¿T0t&ð”Ã@°]LÜ@N½ Ï‚n¦KâÀ«=û‡¡2Æª!ŽË@ù�UÏMsªœ+àú£ƒêŽÝŠêøÊoøkˆ|š|ú„„áŸ;<ÓÅÇÄÍ1-!10Q3áŒ&«¾œÛˆÂjÝóekÇ}±`�WêÌ&†LüDµ­X…}%Êg³D˜ð‹ºm*üÈ!}Or®1W0R0Æ…§kõó ÝuàÀÕÒÊ8h2�~ô¸?�ÌòÍZ,ÃVìÏ^=2"Æ	Ã,SâTÃÝn»3\ËHiy¼àaôöŽ S��åŠðÊjèbîGmtšj»öpœÇ€Èn6€·ÓVÌâ“ö”®.Ã2ã.•›·³[V´ñ“ÀPÇ�#Ø¼*µÕö5c…„²¿xÆ8(õÆÑƒ8Ö3=§Ä¼3(#nÂHqÆ|QÓ¶)›óÊ_}µt¬øà_L�ó£�{»@ô0…[N(ü»x!TU}þýg5|û¿ÌÊÚXÓƒqÐ«ñàTÀÒ5Íib“·¶�ßô:�êc¯ÉŠ"¢]jÝÐWNaöpkx<²©×,‡€Uþ§õélú4Ý¿ú³Óƒ%“Có|lþ¿](Í>X3¨ø*ðßE0�NŒn¾ñž,²ÿµx4cp§ÏÛ�õÉ~oÚpµ¼ò¢¿pE¼p(§³·jX¡…Nt=Å¦ª2�       ’  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  !Ü<?xml version="1.0"?><Features nextValidID="16"><Feature recentID="15" name="LATS1" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="936-4328" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="14" name="lacI" directionality="1" translateFirstCodonAsMet="1" translationMW="38559.52" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6688-7770" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli. &lt;/i&gt;This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="13" name="AmpR" directionality="1" translationMW="31516.09" type="CDS" allowSegmentOverlaps="0" cleavageArrows="4815" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment name="signal sequence" range="4747-4815" color="#ccffcc" type="standard" translated="1"/><Segment range="4816-5607" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="12" name="GST" directionality="1" translationMW="25499.14" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4328" maxFusedRunOn="4328"><Segment range="258-911" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;glutathione &lt;i&gt;S&lt;/i&gt;-transferase from &lt;i&gt;Schistosoma japonicum&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="11" name="ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5778-6366" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="lacZÎ±" directionality="1" translationMW="19741.77" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="7893-81" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;LacZÎ± fragment of Î²-galactosidase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacZ&lt;/i&gt; fragment&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="9" name="AmpR promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4642-4746" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="lacIq promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6610-6687" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;In the &lt;i&gt;lacI&lt;sup&gt;q&lt;/sup&gt;&lt;/i&gt; allele, a single base change in the promoter boosts expression of the &lt;i&gt;lacI&lt;/i&gt; gene about 10-fold.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacI&lt;/i&gt; (mutant)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="lac promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-35" range="7819-7824" color="#ffffff" type="standard"/><Segment range="7825-7842" color="#ffffff" type="standard"/><Segment name="-10" range="7843-7849" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="tac promoter" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-35" range="183-188" color="#ffffff" type="standard"/><Segment range="189-204" color="#ffffff" type="standard"/><Segment name="-10" range="205-211" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;strong &lt;i&gt;E. coli&lt;/i&gt; promoter; hybrid between the &lt;i&gt;trp&lt;/i&gt; and &lt;i&gt;lac&lt;/i&gt;&amp;nbsp;UV5 promoters&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="CAP binding site" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7783-7804" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="thrombin site" directionality="1" translationMW="627.76" type="CDS" allowSegmentOverlaps="0" cleavageArrows="929" consecutiveTranslationNumbering="1" maxRunOn="4328" maxFusedRunOn="4328" detectionMode="exactProteinMatch"><Segment range="918-935" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;thrombin recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="lac operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="219-235" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="lac operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7857-7873" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="M13 rev" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7881-7897" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="7913-7929" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  –<Notes>
<UUID>df309827-8a3f-491e-8694-d1d088504390</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pGEX-4T-2</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="16:0:0">2016.10.14</Created>
<LastModified UTC="1:20:25">2021.12.20</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  )      õ  j        jr›7ßXëƒ-=°Sˆ&#BÉ[xÙk"ÎÔ3{ò¸bªŒñÛeÂ¸5f¸VŠim°ì™b Z!0eÖ­ÝtšØ¿æy
hi@ú#_ÞÇ®¨n…Y…j&1…e`ÅuT¨Z J¿[¯¦™¡‚¥iwK˜=¶r™“‡SuØ  ¢õQ24F­c¡A0ªiÂ*ÄZ6d•¡^íR†«¤ÔçáXáV+l©‹e]š•‚b_Ûáëb‰‡;¡Š*™ØÖ#àc+õZJŠ¥UÉ¡NoÝZIèVôÔ™“`ú�ƒSÜ¨ ƒo½VÁ¸+Ì�¿ÐZüÄÃÍ²n>ÌÊ°†!WîØÓ¿p·N‹‰LðGSòÐ3-0"ƒ,4öÒÜ“0H™k¡Gì†sæªd)bØe–
ºG-7ªÀˆÌþj*j®Vvµf&ó¶Ö_á•Žµ–hUAD˜I­v¯"Ïé¯ê¨Ãj�¯Š¨íýÎT•F8Q´ #q )Û�»]0”o¡«?Å*½a„Þ©R¸æ Ì&›ò(sÀÔM{<ÙÞk´Xz
{}-ÑTÛ§‡IDZcHãÙ}„†]ã^ž¸uÆÁÅåóª£uØ m‰{>†Ê|……=ŸWL‡}Æ>4ÖuÊ3Wš^^z{“¯Ã]idxÁ•(Ð}ëŒì?¯
š8enð„È³	¶�7€=%?¿&É)¹Üp´5æ‘Ñ³„d#íá¥6‚^-R§¶fz‘¡©W­ª–¥PnBoª†[¶U¿¥ÈJ¬¯xL´ÉFÕë¡êªã2[Ž:5uU}óŸL ¨¨ðmDç×º«Å	x:ÓDÍõg‹ë’$òJCßzÔ^âMóçgìî9oŠ} ã�H1”².Ÿ�íG)ðeÓ…À²7tý:ÅÇç|^Ÿ�2˜EL÷:üPPÁÐ›ÂSÁt3q:ô W>	º™/‹¬÷î„Ë¨†;- æW=5Ì>ªp¯ƒêŽª;v+«ã)¿á® eòióê†|ìóO4Ä´„ÄÁDÏ†0š®úsn#	«wÍ•¯öÅ‚5_«0˜63ðQÖ´!bô—)œbÍcÂ.é´|«ó …ô==Ê¸Å\ÀUHÃ0N�¯×Ìƒu×ƒWK(á Ê=ûÒàþ73Ë5h³i[³<mxôÈ ‹$\S±O‰S#uºìÍs- 8m¥æó�‡ÐcÚ8�N>7–+Ã)«¡‹¸}ýµÒiªïØQÂs M¸,ÚßM[3ŠLÚR¸»ËŒ¸886VnÞÍmZÓÆLC�bðª×WØÕŽ6Êýãà£×Fà3XÌöŸð8Ì Œ»	 ÇñGNØ¦oÏ)}öÕÐj³ã€5}2ÎŽ5îíÐÂm8£òíà…QU÷ø3õœÕóîÿ3(<kicNÇB¯Ç�SHL×5¥ˆJNÞÚCÐêw©Ž¼;&(ˆˆLu«wA]9‡ØiÁ­àòÊ§\²Wø2Ÿ×¥³èÓvÿêÏN–LQÍñ³úýt£4øX1`Î£à«Ã}Â<881ºøCÆx²ËþÕàÑ�Âœc?nw×%ù¿iÂÖóÊŠýÁñÀ¢žÎÝ©b†89ÐPôsš¨`Ê
      i  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
   ¦<?xml version="1.0"?><Features nextValidID="15"><Feature recentID="14" name="lacI" directionality="1" translateFirstCodonAsMet="1" translationMW="38559.52" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="3319-4401" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli. &lt;/i&gt;This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="13" name="AmpR" directionality="1" translationMW="31516.09" type="CDS" allowSegmentOverlaps="0" cleavageArrows="1446" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment name="signal sequence" range="1378-1446" color="#ccffcc" type="standard" translated="1"/><Segment range="1447-2238" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="12" name="GST" directionality="1" translationMW="25499.14" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="974" maxFusedRunOn="974"><Segment range="258-911" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;glutathione &lt;i&gt;S&lt;/i&gt;-transferase from &lt;i&gt;Schistosoma japonicum&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="11" name="ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2409-2997" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="lacZÎ±" directionality="1" translationMW="19741.77" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="4524-81" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;LacZÎ± fragment of Î²-galactosidase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacZ&lt;/i&gt; fragment&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="9" name="AmpR promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1273-1377" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="lacIq promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3241-3318" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;In the &lt;i&gt;lacI&lt;sup&gt;q&lt;/sup&gt;&lt;/i&gt; allele, a single base change in the promoter boosts expression of the &lt;i&gt;lacI&lt;/i&gt; gene about 10-fold.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacI&lt;/i&gt; (mutant)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="lac promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-35" range="4450-4455" color="#ffffff" type="standard"/><Segment range="4456-4473" color="#ffffff" type="standard"/><Segment name="-10" range="4474-4480" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="tac promoter" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-35" range="183-188" color="#ffffff" type="standard"/><Segment range="189-204" color="#ffffff" type="standard"/><Segment name="-10" range="205-211" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;strong &lt;i&gt;E. coli&lt;/i&gt; promoter; hybrid between the &lt;i&gt;trp&lt;/i&gt; and &lt;i&gt;lac&lt;/i&gt;&amp;nbsp;UV5 promoters&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="CAP binding site" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4414-4435" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="thrombin site" directionality="1" translationMW="627.76" type="CDS" allowSegmentOverlaps="0" cleavageArrows="929" consecutiveTranslationNumbering="1" maxRunOn="974" maxFusedRunOn="974" detectionMode="exactProteinMatch"><Segment range="918-935" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;thrombin recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="lac operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="219-235" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="lac operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4488-4504" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="M13 rev" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4512-4528" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4544-4560" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  –<Notes>
<UUID>df309827-8a3f-491e-8694-d1d088504390</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pGEX-4T-2</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="16:0:0">2016.10.14</Created>
<LastModified UTC="1:19:12">2021.12.20</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  ¤ý7zXZ  æÖ´F !   t/å£áEa] Ë‡ØÎf‘ƒÊý{3Ôé·Ú(1v%f M*	mj÷)p80çÌ*iÚ	´NY]Qó“�úF7î&ƒÕ!Ò÷ÀnûÌ\¥ÀÄyfÂ ¸êyaÉ ÎLGŸÿ_u±	”½&Fy7•ÜKQ¶íYÙìE_p2™´×'ê®(fr�üÆã°ª¡tWÛš}ðn& *ÑÇøK0ÁßÅÌDª©ÿŒ‚ÜSn±aiHäØ95�Öë�G‡l4	�ìò_ÅËÏ'B›y×ª"ŽÊƒ‰¹!ð~ŠvÜ˜ &o¦¤g²yS;‡a¢]ÈÑ,1wÌ‰(åœ°‘ÌMÖ
”§·r[WŒ´G¹`˜§“s=]Yxrpfý•¸I{ãèû´ïª¢Ýžv¼Ïó¬Í¤½¡U]Æ!õo’êä®ÃRYo¥}9|°«Ú”Õà/ßÀ#˜<qenS~¥¶®N|C%,‹	¯vÄO¢}]Õ|Þy¨Ñï¡ÍÅoÊ&d3Šà¶ÆÉ¾oÝ•?õƒü{!ü8ƒICN,ºêÕ±P£Æ5ps×Ó5Gó0N�OJ*#¢ãµÄ÷HàwlˆÞ(]üME*|•®Ø%#Ør€¤»ÇŽª}¨JºýÐÞôÌåAîñÝ›a9hÎd”G 
)öïŸÿ9�‰¯9@E½[­zŽ Ó$MgôÜƒË{QE²4]Íç^þ§¿¢[rÃ¸5Ñês9ÇMlHù¤J$oø³šíˆ:‘1Û0ç6òDñïùÍB©oëíÓQDáƒ¯µ2ùœ!SÎ}Õµ*íÓâÊ®+B®Öxy�óÆÒ&;#çSeÿ¥GJ±�×Ï˜`Œ‹8ÆqY*=Ó~oœ-˜:	ùÇ.Ôqc_~õWQ
…gâ|(D¹üY
[QpÅ/þ!¨‰rpt¶¼ÃU¹ª+l�KÉ, “Ory&À-÷bZî5·±�_¿9Þ|ÐžµNjU�3+S*Ú©1�ƒüãK›@›4¸*Dvr~·—ÌsøN 0Å—ÖRÆò,EÙqRÙôhMªŽÂÿÌ`¡ˆ•™‰ÝˆvÛ ôé¯³o£&q¸1ùCÒèÒ•zá¯t¿W¸XÎºw,¤&Ã=Ð¦ZÔ9ôP×U1?ygb�Ùåb·9²5Ô�Tý÷@BÏ«€¸ÂºSYlD“«’2(Ì�¸þ�ˆvOÉþJ8fzƒŽ‡¥H
fÚ¬]¡�œG9×N­]d¶û¶ÄVœFø²Ç<“;$Éiðìvç)vÍniïQ6/—ám-÷Ê7é •UVo‡Ø(Œ,KSkþ^¾ÓSB…Ï°6!)‡ñDé
}÷¸‘ák‰¶VüagÍØ}B¡
Ã8gÍ®•ÁÖÅ£Q³iWßlƒŽt‰t„¡
L$]æiã’Ø›éhïÊ9ÂÒP‡:"3;1ùL?ž‹RE”ƒ“¶j=íúG?âýnO[�IV@Ý¾�dÄ ü”~ËæìþÑ#é-sæQ6¢àS7TÐ 8XSy$$['µ×S=?è"4ò…—Ê
R*\»ç¡»Ñ!ÝLßá‹¤øí¹8½BEÜãh:ÂR%ÁlÆlA–>¿Gã:S¬ŽíêD›ã²¢Ê·K®Ðž'ÍS&÷úÔ@š £Îî
ÔXË[“$Uæ:#ÇƒÁð}©Ý±Í´1¨‚•ÄÏË»X4Gd«×´r8ŸQQÕ/I:úßA¶÷Ù¤�Ñ­Á	¶´áÁ~Ðš¯åÛYY˜vŸû«kkë…„‚3F
Æ[{áº™åŸRš–6áõR—+,0¬H”£‰¥Ißž8LÞ.Ë²r€oÇR
ò¡d^*ÿT9s¾—TAkÚ{zÛ½?V@ÆYü×6þß�ØÝ°ù�0†‰æ*Â�'^jzÒ»üÞÎeV:ŠYÇ:-\¤jËŒiH¬éVNu€‡¡êÁh©~8Ì›#[Š¾%×&½“Ñ»‡ 5ôjÒŽdÌC'ýr+W¦åmè`uÂ{Fp)6ÃëŸì=ñt³~í{‚Už  "±ÖÏò~�‚oƒ¨]è=RH8ÉrÓ{ƒ·BnY@aºL›º_Šª¾Ï¡?M´½©²±I-Á25F'b!jDNŽÙµjÝý¡ÐLÅq¿Ó¸��$Új¿CréZ
¹èë	î†ÀY1õ­�?¾ÅXTÙO+sÚú­°ÀùÆ±gp ¶Ì©Ä‹›G­ïXjsŽkýQ¯ú¼“žû‰Nâý{UpG¨ÄÛSo7Õ)Ô~1²(ÂŠá�ýð5Xòº§…ì·½9YŽãÃÈÄ
1r?,]’áË?_Ù¹=?‚“æœ<¥�¤íæ^0îr—ùýožË-ƒt·?¯ÙL…ûœ4nSæÒ®ÅãM�¤ÅF#¸æÖñêA<À-ÑË‰5‹M¸¹L‹°U4í‰�÷èø¬Jû¾ÔšÃY•™UoÚ;©\+8™w bó¼�î�[Rë–rù~?Ü…>øxöì�>b�…Q´è‰OÏžêíÜ_[?oØåH:§RK{·EžM×xÀ•Dõ!3J¥S�÷rJÃG÷ÖC¥üû][ÛÖÔÿþKÙ=š+‚­#D­V3]lM²Y|ù|iÜ¯ª—Œõh2_?‰eî@Î•0%É‰Jsjfœ†ˆ¼époáCF’ö?—ƒÎõ�Ê×TN¡yé�{Äµi(ï &ÊýÞËEd„c<‰x1âi™È’G÷|sëRD_ghŸj™à5ô€oêþØ†¶Í6Í“¾7Oª 7h~¹zÐöX‘ÌïZ�Ò‘Î�—dä'«rãÎžýYˆW ä+$b]dDF^mR€‚›¸Â©2ñé{Ç#/…‹±�ŠHžÑÏMü‹„Ÿ&ì8�Ê{xü «o½®rÏávgRÓÁ7À¶'�‚g°¡›`ä9^Ÿ6æeÝKX›ç´!Ç÷T…X¨½½i¶žï?Êø¹ÑT ^ ²“RS7Ew~±L¯ý¢º*ª$ßp6ËE¦Ê”9ÛÀŒ¡ÊÝ®ëRI·™†…õ
Á„#\¾�á$	ÒØt—ôQP}š0¤"™NŽ+}õ.˜iYŒwœÈ J3ªa–èsú~(Ì/à/qvSKœÂÜiûÀwÑ¤)òå´ÀyŠšÚÃ²‡Ÿ�:×æœþßŠÈÿ–,äÍn�œÓ‡Šl=Çð]Êç�î£vâ÷mL¹ý;Hô ”˜‚Ç=S˜•”Žñ@×€Jæu5e‰L$§ç %b}/«ÖÞ{®Ó4·'à¦#×‹§í›=s@Á|>Ûõ'’k³d¦™%c8¶×ÉÈ’vêa2÷î	Þçüí¯”NüÌøq7˜{�R0R¥ÆLÐIúÆCÆÇõléê•¾_+MÆ—©±ÂëlÂ{»‹Þ±9úé¦‹Å±·²‰§‚õ½$ÝÕŒ†ZÍ"'zêìüÚ““…@²Í!%îz›`uAºm=¸Îýú½üÊ—Z�­òn‚T»1”<ðèå’0+GWº¼X �fÖ@Iû�FÜ»˜µLBÑÑ	^ˆP ²(+†¾ñd¾ö·��Îšæ¦hrÓ Ñï=
ÌÚ2®jôño
N\›ÐqøàÉÓB\
Z- Ý¬H&äJ:¯zá8¯i^ËŸ½YÞî”¥^°Ó¸ '<ðtƒ5j?ÏË¡»ÉQ×
ßLUº^N«í6[g²¿Ç>&Ÿûf¹ù”ØñDF(`®‰YÈî–:,:Ý¬!#ƒø~{§W´³Ù¤›R�æŠ%X[þ¢9‡2³¬¤w=—�ds[‹[økþ¶“ÜÖ�Ç/ß|óº=š¯9“<³ä}ûìî‚S�ÇeÈÁ“jh\Q1x¬½ó‰Ó[$	dÃ˜í•Iµ‘´­Œ¹øR–’�Ãjã½öê—�cRÙjÍ±*Öñ˜­\éƒ¨]ýÇ�¸~‹Vþ?—Š&»vm¹«jy;!"}FQIáÑµ	�¶WäŠUßìu5Ž#)ÓÙ¢rä­'z:$çÉH-iNó	­sÊsâ˜¥.[GSE³ÔÝsL_ j3|åýê~F='Ã½—À¼Ø_ ¾�tÏ7ãõ‡“rˆD\ó°"ëA×ÊÉq,Ðä y²LSX¢1	jD®-ø±ýÀ¿"5É“C{:¢Ã@n)ñ	ÒÖ¼yMo!1O¶!Ì÷JžV/GHœ‹Ç±9 ƒÅYß"›�½×é?”�åt¥]~÷÷r_‡îÉŽÇWhÒ½¬6ŠféW¥õ>,5±çŽ¢|_h?¶M˜”%×–¡Ù—.E”]QWÀ×ZÈÁY­w­ŸGVT"/on‰ÐÊ—A/ÍrÎùV0î3†h_+CKºh ÃŠU [³�g VÂ¿9™4v‚§žæL¡¸fz¦Â$iÚ¯¹áYUøz^¼€ºgI¨qStïêÑµ�ï?Xßÿ.€)v2%]nÌçëÆ"Œ$nYõ�ÒçžÎí°´q&êwÜUž×èzWG±ÔžJ5oŠŠô1;{ûÈ6>¯¿ÒY-³Å¦{Èt,±n@“„ôÖÊ¶Ýª¶$e@Z¶ú*wƒúËRZlïÍhÃ÷û�.D|dÀ9s@*+ÿ9–~PÁ¯/ß�YÞý…¿‘-¸ :Lˆ¾­Ê)§=•7]<½”2Ì.&UÊŒôžŸ­—v÷ã¹ù)ãdEa¿wS$ãFˆúÂ£¥‚¿ˆý¬Å;MX÷h„¨î¬°ÞœõƒPb×¢-8KàõØúŠÆ2gÂwH<Çëåÿl4rÊNŸ9/‘[OŽ_-¨5 šcxŽ®FÓ…ôü”n]ê³…>‡ïýRŽ^ìõpè¬
‰‘Èß_ÂTNá
šF‹Î’ƒùÊ}ñ Œæø‰ûjÎÇ†üÂ×	¨Å`šóƒÕ¹Úð|¼—–‡ŸWÏò!$½HÌf´³Ã‡S@m!ÎßHâœeW(¢4ˆw;æS|
€œXm-ü,{
Íc•ŠT³À$9Ä’2*Z6öæ,ŠTÒP$ÕÃ°Üy•²èb8†÷0âòî™�¾õXf<“ŽŒU)�q!`éÐå8ŸØÊÚCÐuz€döÄ$/†jGº#¤sÎ´�L4¦¡³1W°)EV`Ûîý˜ï½p®f¶sÚÌDž+ÓÐ’àºFÙæÜqCÈÌ Œâ¦³� Ô&<r’ÖNÙnýÄFZéºJ¼<Ç›†¸}.È‡¡¹‡3á7@�‘„îÍÈbž>ÄÞÄ½� 0ck¯Øè‚ˆžN æœ"©Óâ›%ÑV	ÈU’øÄ�My}GÏÀÞ}ÅE-)ËàÓ‘ïÕuU–b(ærýè€Ë?"¦yàO0‰î‡ºü¢ÒÊ¡zÚ¸`æa£Cý“ú Bwä‡óq“HiÍ¹Ÿê;e¹J�ÕëµäèQG×ü,<¨OsÓèn'k�´e¯”7–…»d·7fG}æ´Žx.q…j:Ædðµˆ]ÞõêQþ‰yèd;Š†ûåÊôðµƒÓ™Ãkt…¤Gâr\X*üÎHkí{qY&ºêÓŒê”ys¢Pžäú…p      ObÇFå ý Æ¤ |p£B±Ägû    YZ   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  -<?xml version="1.0"?><Features nextValidID="17"><Feature recentID="0" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="7265-7281" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="M13 rev" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7233-7249" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="lac operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7209-7225" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="lac operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="219-235" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="thrombin site" directionality="1" translationMW="627.76" type="CDS" allowSegmentOverlaps="0" cleavageArrows="929" consecutiveTranslationNumbering="1" maxRunOn="3680" maxFusedRunOn="3680" detectionMode="exactProteinMatch"><Segment range="918-935" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;thrombin recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="LVPRGS"/></Q></Feature><Feature recentID="5" name="CAP binding site" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7135-7156" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="tac promoter" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-35" range="183-188" color="#ffffff" type="standard"/><Segment range="189-204" color="#ffffff" type="standard"/><Segment name="-10" range="205-211" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;strong &lt;i&gt;E. coli&lt;/i&gt; promoter; hybrid between the &lt;i&gt;trp&lt;/i&gt; and &lt;i&gt;lac&lt;/i&gt;&amp;nbsp;UV5 promoters&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="lac promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-35" range="7171-7176" color="#ffffff" type="standard"/><Segment range="7177-7194" color="#ffffff" type="standard"/><Segment name="-10" range="7195-7201" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="lacIq promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5962-6039" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacI&lt;/i&gt; (mutant)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;In the &lt;i&gt;lacI&lt;sup&gt;q&lt;/sup&gt;&lt;/i&gt; allele, a single base change in the promoter boosts expression of the &lt;i&gt;lacI&lt;/i&gt; gene about 10-fold.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="AmpR promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3994-4098" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="lacZÎ±" directionality="1" translationMW="19741.77" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="7245-81" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacZ&lt;/i&gt; fragment&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;LacZÎ± fragment of Î²-galactosidase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVGITLSTARCTNASGVRQPSEAVVWLCRS*"/></Q></Feature><Feature recentID="11" name="ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5130-5718" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="GST" directionality="1" translationMW="25499.14" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3680" maxFusedRunOn="3680"><Segment range="258-911" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;glutathione &lt;i&gt;S&lt;/i&gt;-transferase from &lt;i&gt;Schistosoma japonicum&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"/></Q></Feature><Feature recentID="13" name="AmpR" directionality="1" translationMW="31516.09" type="CDS" allowSegmentOverlaps="0" cleavageArrows="4167" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment name="signal sequence" range="4099-4167" color="#ccffcc" type="standard" translated="1"/><Segment range="4168-4959" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"/></Q></Feature><Feature recentID="14" name="lacI" directionality="1" translateFirstCodonAsMet="1" translationMW="38559.52" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6040-7122" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli. &lt;/i&gt;This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="VKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"/></Q></Feature><Feature recentID="15" name="SHLD2" directionality="1" translationMW="101777.12" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3680" maxFusedRunOn="3680"><Segment range="936-3647" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSGGSQVHIFWGAPIAPLKITVSEDTASLMSVADPWKKIQLLYSQHSLYLKDEKQHKNLENYKVPESIGSPDLSGHFLANCMNRHVHVKDDFVRSVSETQNIESQKIHSSRLSDITSSNMQICGFKSTVPHFTEEEKYQKLLSENKIRDEQPKHQPDICGKNFNTNLFQLGHKCAAVLDLVCSTEKINIGPEVVQRECVPTEYHEIQNQCLGLFSSNAVDKSRSEAAVRKVSDLKISTDTEFLSIITSSQVAFLAQKKDKRRSPVNKGNVNMETEPKASYGEIRIPEENSIQLDGFTEAYESGQNQAYSLELFSPVCPKTENSRIHINSDKGLEEHTGSQELFSSEDELPPNEIRIELCSSGILCSQLNTFHKSAIKRSCTSEDKVGQSEALSRVLQVAKKMKLISNGGDSAVEMDRRNVSEFKSIKKTSLIKNCDSKSQKYNCLVMVLSPCHVKEINIKFGPNSGSKVPLATVTVIDQSETKKKVFLWRTAAFWAFTVFLGDIILLTDVVIHEDQWIGETVLQSTFSSQLLNLGSYSSIQPEEYSSVVSEVVLQDLLAYVSSKHSYLRDLPPRQPQRVNSIDFVELEHLQPDVLVHAVLRVVDFTILTEAVYSYRGQKQKKVMLTVEQAQDQHYALVLWGPGAAWYPQLQRKKGYIWEFKYLFVQCNYTLENLELHTTPWSSCECLFDDDIRAITFKAKFQKSAPSFVKISDLATHLEDKCSGVVLIKAQISELAFPITASQKIALNAHSSLKSIFSSLPNIVYTGCAKCGLELETDENRIYKQCFSCLPFTMKKIYYRPALMTAIDGRHDVCIRVESKLIEKILLNISADCLNRVIVPSSEITYGMVVADLFHSLLAVSAEPCVLKIQSLFVLDENSYPLQQDFSLLDFYPDIVKHGANARL"/></Q></Feature><Feature recentID="16" name="Myc" directionality="1" translationMW="1203.34" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3680" maxFusedRunOn="3680" originalName="Myc" originalSequence="GAGCAAAAGCTCATTTCTGAAGAGGACTTG" isFavorite="1" detectionMode="exactProteinMatch"><Segment range="3648-3677" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Myc (human c-Myc proto-oncogene) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="EQKLISEEDL"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ¥<Notes>
<UUID>5eb419d2-c8cc-4f54-abb0-5b2435e71445</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pTac-GST-SHLD2(human)-Myc</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="16:0:0">2016.10.14</Created>
<LastModified UTC="23:28:15">2025.9.4</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y      K         
?????                                                                                                       ÿ d      DO  d              E              ÿÿÿÿYô? P                                                                                                                                                              3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
   )<?xml version="1.0"?><CustomEnzymeSets/>
